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As plants are very heterogenous and contain many different metabolites like polyphenols, polysaccharides, or acidic components, NucleoSpin® plant II offers two different lysis procedures for optimal processing of various samples. We used the PL1 lysis Buffer based on the CTAB lysis method. In addition, the silica membrane in the NucleoSpin® Plant II Columns is optimized to improve DNA binding, and NucleoSpin® Filters are included for convenient clarification of lysates. NucleoSpin® allows processing of up to 100mg(wet weight) or 20mg (dry weight) starting material,with typical yields in the range from 1 to 8µg DNA. The eluted DNA is ready to use for PCR reactions. The CyBio®-SELMA is a semi-automatic parallel pipettor which works fast, precisely and in a high reproductive manner. Since pipetting to 96 wells simultaneously is advantageous for automating NucleoSpin® 96 Plant II kit, the SELMA was evaluated for its ability to speed up plate processing compared to using an 8-tip manual pippetor.
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In the Life Science technology area Analytik Jena is pooling its biotechnological competence. It provides a wide product spectrum for automated total, as well as individual solutions for molecular diagnostics.
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