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Standardize tumor sample preparation and reduce background with Curiox laminar wash system

Aug 10, 2022

Interactions of cancer cells with their environment profoundly influences tumor progression, ultimately determining whether the primary tumor metastasizes, develops dormant micrometastases, or is eradicated. Thus, the tumor microenvironment (TME) profoundly shapes therapeutic responses and resistance. Tumor-infiltrating lymphocytes (TILs) possess the unique ability to recognize, attack and penetrate tumors. As such, mechanisms that unleash the power of TILs are under investigation as potential cancer treatments. For instance, mouse and human TILs are used in the preclinical studies of immunotherapeutic agents, while other human TILs are being developed and applied into novel immunooncology methods.

Hence, TIL isolation and further characterization are of key importance in assessing treatment efficacy in various tumor types. However, the downstream processing from TME encounters a common problem. Typically, after tissue dissociation and isolation, TILs share their samples with high amounts of debris and dead cells in solution, contaminants needing stringent washing for removal.


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Curiox

Curiox Laminar Wash technology provides an advantage to scientists in processing suspension cells for flow cytometry, eliminating the usage of the centrifuge.

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An affordable, benchtop instrument for flow cytometry sample preparation

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