Products

Naica System - Stilla Technologies

For Crystal Digital PCR

Crystal Digital PCR is Stilla’s next-generation technology for absolute quantification of nucleic acids. Using cutting-edge microfluidic innovations, this technology integrates the Digital PCR process in a single consumable.

The sample is first flowed through a network of microchannels and partitioned into a large 2D array of 30,000 individual droplets, also called a dropet crystal. PCR is then performed on-chip and the crystal is imaged to reveal the droplets that contain amplified targets. The last step consists in counting the number of these positive droplets to precisely extract the absolute quantity of nucleic acids.

With Crystal Digital PCR, the combination of powerful image analysis and intuitive visual inspection offers an unmatched level of confidence in the digital PCR measurement, yielding data you can truly trust.

Key Features:

  • Best ease-of-use in digital PCR
  • Fastest time-to-result (2 hours)
  • Unique 3-color detection capability
  • Crystals of 30.000 droplets are created from each sample using microfluidic technology
  • PCR amplification is performed immediately after crystal generation
  • Crystal is imaged to reveal the droplets that contain amplified targets
  • Crystals are read using LEDs channels (blue, green, red) scan up to 12 samples in just 10 minutes
  • Software automatically counting the number of these positive droplets in each population converted into absolute concentration of targeted DNA sequences

Key applications:

  • Absolute quantification (DNA/ RNA)
  • Copy number variation
  • Rare event detection
  • Gene expression
  • Single cell analysis
  • Whole cell amplification
  • NGS library calibration/ NGS results validation sequences

 

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https://www.stillatechnologies.com/naica-system-workflow/
Katarzyna Nazarewicz

Sales manager

Katarzyna

Nazarewicz

+48 797 701 027

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