Transcriptomes
Digital PCR
High sensitivity and precision, reduced hands-on time and a 3-color target multiplexing
Digital PCR relies on partitioning a sample into numerous distinct components within which the PCR reactions then occur. The scientific community quickly recognized the unique advantages of digital PCR over traditional endpoint or real-time PCR: the assay does not rely on a standard curve, has improved accuracy, provides absolute quantification and offers improved detection of low copy-number variants.
Other key advantages of digital PCR became evident later as the method was used more broadly: reproducibility over time and across different labs, robustness and tolerance to PCR inhibitors.