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“I used the xCELLigence DP system to study the effectiveness of CAR-T against target cancer cells. I was able to observe the effectiveness of cell therapy during the entire experiment with great accuracy. Both devices and software are easy and pleasant to use”

Dr Anna Bujak, Principal Research Scientist

Helix Immuno-Oncology, Warsaw, Poland

xCELLigence SP - Agilent technologies

Basic tool with a huge impact for any cell based assay

The RTCA SP Station is located inside a tissue culture incubator and is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement. Under the software of the RTCA Control Unit, the RTCA SP Analyzer can automatically select wells for measurement and continuously transfer measured impedance data to the computer. Cell Index values, derived from the measured impedances, are continuously displayed on the Software user interface.

The RTCA SP Instrument is composed of four main components:

  • RTCA Analyzer
  • RTCA SP Station
  • RTCA Control Unit
  • E-Plate 96

RTCA SP Instrument Highlights:

  • Rapid measurement time - less than 10 or 60 seconds for 96 wells
  • Compact design of the RTCA SP Station that fits conveniently in tissue culture incubators
  • High-performance electronic components and enhanced instrument reliability
  • Easy setup and customization of assay protocols
  • Integrated, on-line data analysis capability

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https://www.aceabio.com/products/rtca-sp/

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Cancer Immunotherapy Applications of xCELLigence Real-Time Cell Analysis (RTCA) Systems xCELLigence cancer immunotherapy: NK cell-mediated cytolysis Real-time chemotaxis of macrophages Quantifying virus-mediated cytopathic effect with xCELLigence platform Introducing the xCELLigence Immunotherapy kit – B Cell killing assay Real-time monitoring of Human Skin-Derived Precursor (SKPs) cell differentiation Assessment of CAR T Cell killing capacity against solid tumors-Thursday, October 20, 2016, 5 pm CEST Cancer immunotherapy: Macrophage-mediated phagocytosis Monitoring in real time the formation and removal of biofilms from clinical related pathogens xCELLigence in personalized cancer medicine Investigating the hallmarks of cancer using xCELLigence system Monitoring the formation and removal of biofilms from clinically relevant pathogens in real-time Real-time determination of virus titers using cell electric impedance technology Label-free monitoring of T cell activation by the impedance-based system A novel high throughput assay for diagnosis by real-time monitoring of parasite motility Optimization of (CAR) T cell design guided by sensitive sssessment of B cell killing Cellular impedance measurement- novel method for in-vitro investigation of drug efficacy Cellular impedance measurement- response of OVCAR-3 cells to Cisplatin and hyperthermia ACEA webinar series: Using xCELLigence RTCA to study biofilms formation Protectome analysis: A new selective tool for bacterial vaccine discovery Kinase study of cytotoxicity voted paper of the year.
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Katarzyna

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The RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

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