Discover

Back to filter

Related topics

Webinar: Join us for an In-Depth Look at the Revolutionary Small Animal Radiotherapy System at STTARR

Precision X-Ray

Aug 17, 2023

Register for an engaging webinar led by Naz Chaudary, Ph.D., and Research Technician, Alex Wang from the...

Optimizing Gene Expression with Bioluminescence & the piggyBac System

Spectral Instruments Imaging

Aug 15, 2023

Discover how bioluminescence imaging & the piggyBac gene editing system optimize & track gene expression in mouse...

High-plex immunofluorescence imaging and traditional histology of the same tissue section

RareCyte

Aug 7, 2023

RareCyte Orion’ platform has been used for collecting H&E and high-plex immunofluorescence images from the same cells...

Drive key insights and discoveries with NEW Live Cell Analysis applications for eSight system

Agilent technologies

Aug 2, 2023

eSight is now powerful like never before. Learn more about new dedicated application modules for live-cell analysis: 3D...

Single cell-resolution in situ sequencing elucidates spatial dynamics of multiple sclerosis

Vizgen

Jul 14, 2023

MERFISH integrates spatial transcriptomics technology with high resolution spatial imaging, fluidics, image processing,...

High speed, compact and fully automated microscope inside your incubator? Lumascope850

Etaluma

Jul 13, 2023

The powerful, new LS850 Microscope is the latest generation of our fully automated three-channel flagship model and...

MARS - High Efficiency Separation of CD34+ HSC from Mobilized Blood

Applied Cells

Jul 12, 2023

MARS platform provides an easy and cost-effective protocol for CD34+ cell isolation. Single pass CD34+ HSC enrichment...

43 markers, ONE tube : Impress yourself with Cytek Aurora Spectral Cytometer

Cytek Biosciences

Jul 11, 2023

Using Cytek full spectrum flow cytometry, scientists at Hamad Medical Corporation, developed a 43 color panel to...

InAlyzer - The technology chosen by NASA

MEDIKORS

Jul 10, 2023

MEDIKORS's InAlyzer was finally selected as the equipment to be used for NASA's space environment biological research

Nature Article: The genomic landscape of pediatric acute lymphoblastic leukemia

Mission Bio

Jun 29, 2023

Acute lymphoblastic leukemia (ALL) is the most common childhood cancer. Here, using whole-genome, exome and...

Show all topics (10)

A novel real time imaging platform to quantify Macrophage Phagocytosis

Feb 26, 2018

Quantification of macrophage phagocytosis in vitro has traditionally been technically challenging. Here we report the optimization and validation of the IncuCyte S3 real time imaging platform for macrophage phagocytosis based on pHrodo pathogen bioparticles, which only fluoresce when localized in the acidic environment of the phagolysosome. Image analysis and fluorescence quantification were performed with the automated IncuCyte Basic Software. Titration of the bioparticle number showed that the system is more sensitive than a spectrofluorometer, as it can detect phagocytosis when using 20× less E. coli bioparticles. We exemplified the power of this real time imaging platform by studying phagocytosis of murine alveolar, bone marrow and peritoneal macrophages. We further demonstrate the ability of this platform to study modulation of the phagocytic process, as pharmacological inhibitors of phagocytosis suppressed bioparticle uptake in a concentration-dependent manner, whereas opsonins augmented phagocytosis. We also investigated the effects of macrophage polarization on E. coli phagocytosis. Bone marrow-derived macrophage (BMDM) priming with M2 stimuli, such as IL-4 and IL-10 resulted in higher engulfment of bioparticles in comparison with M1 polarization. Moreover, we demonstrated that tolerization of BMDMs with lipopolysaccharide (LPS) results in impaired E. coli bioparticle phagocytosis. This novel real time assay will enable researchers to quantify macrophage phagocytosis with a higher degree of accuracy and sensitivity and will allow investigation of limited populations of primary phagocytes in vitro.

Read more

 

 

Scientific paper
Application
Video

Related technologies: BioImaging

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

Related products

High throughput, real-time and automated measurements of live-cell activity, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey