Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
Discover
Related topics
Intoducing spheroOne: Automated sorting and isolation of single spheroids and organoids
Jun 21, 2022
spheroONE is an innovative single large-particle sorter and dispenser which revolutionizes 3D cellular models handling....
Outstanding post-sort sample recovery on the WOLF Cell Sorter
Jun 14, 2022
The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting...
Incucyte AI module: Advanced Label-Free Classification Analysis
Jun 6, 2022
Monitor adherent cell morphology changes objectively to determine live/dead cell counts or classify cells based on...
Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters
Jun 3, 2022
Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters, whereas hydroxychloroquine...
Intoducing cellenONE: The revolutionary Single Cell isolator
May 25, 2022
A revolutionary platform based on sciDROP PICO precision dispensing technology and coupled with advanced image...
Advances in Leukemia research using shear flow and Bioflux system
May 24, 2022
Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes create disruptions in the immune...
May 4, 2022
A prodigy incorporating a unique combination of patent-pending innovative technologies that takes flow cytometry to the...
In-vivo webinar: a versatile use of SPECT/CT in the development of theranostic tracers
Mar 9, 2022
Combining (preclinical) imaging and targeted therapy, is the basis of theranostics. In this webinar, Dr. Florea will...
Introducing IsoPlexis Single-Cell Natural Killer Panel
Mar 4, 2022
Because of their ability to kill tumour cells, NK cells are an attractive target in cancer immunotherapy, therapeutic...
Enhanced small particle detection on CytekAurora and Northern Lights
Mar 2, 2022
Aurora system with ESP option, can fully resolve particles around 70nm and from the 80nm PS bead. The new violet laser...
Dec 5, 2017
The integrated nature of cell migration is exemplified by angiogenesis. Angiogenesis or neo-angiogenesis refers to the formation of new blood vessels from pre-existing vessels and is critical for development, wound healing and tumor growth. Endothelial cell migration is an important component of angiogenesis, involving chemotactic, haptotactic and mechanotactic (shear stress) induced cell migration. Chemotactic cell migration is typically induced by soluble growth factors such as vascular endothelial growth factor (VEGF) and its isoforms, fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF) amongst others. These growth factors interact with their cognate receptor tyrosine kinases on he surface of endothelial cells activating signaling pathways culminating in directed cell migration.
In the present study, we used the CIM-Plate 16 with the xCELLigence RTCA DP Instrument to monitor growth factor-mediated migration of endothelial cells in realtime using label-free conditions. The CIM-Plate 16 is a 16-well modified Boyden chamber composed of an upper chamber (UC) and a lower chamber (LC). The UC and LC easily snap together to form a tight seal. The UC is sealed at its bottom by a microporous Polyethylene terephthalate (PET) mem-brane. These micropores permit the physical translocation of cells from the upper part of the UC to the bottom side of the membrane. The bottom side of the membrane (the side facing the LC) contains interdigitated gold microelectrode sensors which will come in contact with migrated cells and generate an impedance signal. The LC contains 16 wells, each of which serves as a reservoir for a chemoattractant solution on the bottom side of the wells, separated from each other by pressure-sensitive O-ring seals.
Brand profile
Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
More info at:
www.aceabio.com