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Download today your IsoPlexis e-book: understanding immune Cell Function In Cancer

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Understanding complex immune-cancer interactions is critical to identifying therapeutic targets, devising novel avenues...

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This technical overview describes advancements in detector technology, and highlights examples where optimal signal...

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AVATAR Improves transfection efficiency with high viability

May 9, 2018

Now you don’t have to choose between transfection efficiency or viability, you can have both. Use AVATAR with electroporation, chemical transfection, or lipofection workflows to get higher transfection efficiencies with high cell viability in patient-derived cells. AVATAR lets you squeeze and stress your cells for improved transfection, followed by healthy recovery and cell growth using two key microenvironment conditions:  atmospheric pressure and O2 along with CO2 and temperature. AVATAR streamlines and optimizes the entire transfection process. Precise microenvironments along with custom reagents for before, during and post-transfection support your cells of interest and improve DNA and RNA transfection efficiency.

-Cell preconditioning: Primary cells undergo a ‘preconditioning’ and expansion step prior to transfection using a specific AVATAR setting for the cell type. This moves cells into a growth phase over a 48 to 72-hour period followed by a transfection or reprogramming step.

-Cell recovery: After transfection, cells are cultured using another AVATAR setting for approximately 24 hours to improve cell viability and transfection efficiency.

-Cell expansion: Another AVATAR setting is used for rapid expansion and to enrich for transfected cells.

 

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Xcell Biosciences

The company provides a revolutionary cell culturing platform that replicates the physiological environment of native tissues to deliver reproducible and biologically relevant molecular analysis.

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The Avatar system can mimic the oxygen and pressure levels of human tissues

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