The company provides a revolutionary cell culturing platform that replicates the physiological environment of native tissues to deliver reproducible and biologically relevant molecular analysis.
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Dec 1, 2022
Cytek Cloud is the new digital ecosystem that supports full spectrum flow cytometry research from panel design to data...
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Practical aspects of an imaging study: live in vivo scanning
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In this webinar, MOLECUBES will present a live in vivo demonstration, showing the entire workflow from animal...
Download the newest AVATAR data presented at CAR-TCR
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Xcell Bio CSO, James Lim, presented new data showcasing improved potency of AVATAR-expanded CAR T cells and highlighted...
RareCyte Orion system advances Multi-modal digital pathology for colorectal cancer diagnosis
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Precision medicine is critically dependent on better methods for diagnosing and staging disease and predicting drug...
Introducing MARS system : revolutionary solution for your workflow of cell separation and enrichment
Oct 7, 2022
The MARS family of instruments provides a breakthrough solution to complete the workflow of cell separation and...
Understand the Cancer Ecosystem based on single cells analysis
Sep 22, 2022
Mission Bio is a life sciences company that accelerates discoveries and cures for a wide range of diseases by equipping...
Automating DNA Extraction for High-Throughput Bacterial Plasmid Purification with CyBi®-SELMA
Sep 19, 2022
The Need to Increase Plasmid Isolation Throughput, Challenges with Scaling up Plasmid DNA Extraction, Automating the...
Introducing RareCyte Orion, integrating Spatial Biology with Multiplex Imaging
Sep 13, 2022
Orion, the highly multiplexed fluorescent imaging system, empower investigators to unlock spatial context for key...
AVATAR Improves transfection efficiency with high viability
May 9, 2018
Now you don’t have to choose between transfection efficiency or viability, you can have both. Use AVATAR with electroporation, chemical transfection, or lipofection workflows to get higher transfection efficiencies with high cell viability in patient-derived cells. AVATAR lets you squeeze and stress your cells for improved transfection, followed by healthy recovery and cell growth using two key microenvironment conditions: atmospheric pressure and O2 along with CO2 and temperature. AVATAR streamlines and optimizes the entire transfection process. Precise microenvironments along with custom reagents for before, during and post-transfection support your cells of interest and improve DNA and RNA transfection efficiency.
-Cell preconditioning: Primary cells undergo a ‘preconditioning’ and expansion step prior to transfection using a specific AVATAR setting for the cell type. This moves cells into a growth phase over a 48 to 72-hour period followed by a transfection or reprogramming step.
-Cell recovery: After transfection, cells are cultured using another AVATAR setting for approximately 24 hours to improve cell viability and transfection efficiency.
-Cell expansion: Another AVATAR setting is used for rapid expansion and to enrich for transfected cells.
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