The company provides a revolutionary cell culturing platform that replicates the physiological environment of native tissues to deliver reproducible and biologically relevant molecular analysis.
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Recorded webinar: Anesthesia Considerations in Small Animal Imaging
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Anesthesia settings and operation of Optical Imaging Systems: methods, animal handling, safety and regulatory...
Introducing Cytek Human Leukocyte Kit, the first ever 15-color lyse no-wash assay
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Immunofluorescence Imaging for Rare Cell Detection with CyteFinder II
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Single-cell selection and cloning are required for bioengineering workflows such as antibody production, cell therapy,...
Flash news - did you know, that you can use Singulator S100 also for plant research?
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In this short TechNote you can find how Singulator can help the data quality in Single cell sequencing - now even in...
Webinar: Spatial Biology and immunofluorescence to study human pancreata in in type 1 diabete
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In this webinar, Dr. Quesada-Masachs from La Jolla Institute for Immunology reveals the image analysis pipeline that...
Advances in Leukemia research using shear flow and Bioflux system
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Webinar: Dose Reduction and Image Enhancement in Preclinical Mouse Imaging using Deep Learning
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In this webinar, ir. Florence Muller (Ghent University - University of Pennsylvania) is presenting two recent studies...
Advance your Spatial Biology, customize your panel design with RareCyte Orion reagents
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Orion system, the highly multiplexed fluorescent imaging system, in combination with dedicated reagents, empower...
Extensive assessment of Cytokine production on the NovoCyte Advanteon flow cytometer
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AVATAR Improves transfection efficiency with high viability
May 9, 2018
Now you don’t have to choose between transfection efficiency or viability, you can have both. Use AVATAR with electroporation, chemical transfection, or lipofection workflows to get higher transfection efficiencies with high cell viability in patient-derived cells. AVATAR lets you squeeze and stress your cells for improved transfection, followed by healthy recovery and cell growth using two key microenvironment conditions: atmospheric pressure and O2 along with CO2 and temperature. AVATAR streamlines and optimizes the entire transfection process. Precise microenvironments along with custom reagents for before, during and post-transfection support your cells of interest and improve DNA and RNA transfection efficiency.
-Cell preconditioning: Primary cells undergo a ‘preconditioning’ and expansion step prior to transfection using a specific AVATAR setting for the cell type. This moves cells into a growth phase over a 48 to 72-hour period followed by a transfection or reprogramming step.
-Cell recovery: After transfection, cells are cultured using another AVATAR setting for approximately 24 hours to improve cell viability and transfection efficiency.
-Cell expansion: Another AVATAR setting is used for rapid expansion and to enrich for transfected cells.
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