Discover

Back to filter

Related topics

Intoducing spheroOne: Automated sorting and isolation of single spheroids and organoids

Cellenion

Jun 21, 2022

spheroONE is an innovative single large-particle sorter and dispenser which revolutionizes 3D cellular models handling....

Outstanding post-sort sample recovery on the WOLF Cell Sorter

NanoCellect

Jun 14, 2022

The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting...

Incucyte AI module: Advanced Label-Free Classification Analysis

Sartorius

Jun 6, 2022

Monitor adherent cell morphology changes objectively to determine live/dead cell counts or classify cells based on...

Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters

MOLECUBES

Jun 3, 2022

Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters, whereas hydroxychloroquine...

Intoducing cellenONE: The revolutionary Single Cell isolator

Cellenion

May 25, 2022

A revolutionary platform based on sciDROP PICO precision dispensing technology and coupled with advanced image...

Advances in Leukemia research using shear flow and Bioflux system

Fluxion Biosciences

May 24, 2022

Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes create disruptions in the immune...

Why use Cytek Aurora?

Cytek Biosciences

May 4, 2022

A prodigy incorporating a unique combination of patent-pending innovative technologies that takes flow cytometry to the...

Introducing Celloger Mini Plus: Automated and compact Live cell imaging inside your incubator

Curiosis

Apr 11, 2022

We are happy to introduce new Celloger Mini Plus, automated live cell imaging system! Click the video below to find out...

CQ1 live-cell confocal system: revealing the pathophysiology of COVID-19 infection

Yokogawa

Mar 21, 2022

Yokogawa decided to support the world’s fight against COVID-19, scientifically cooperating with research facilities...

In-vivo webinar: a versatile use of SPECT/CT in the development of theranostic tracers

MOLECUBES

Mar 9, 2022

Combining (preclinical) imaging and targeted therapy, is the basis of theranostics. In this webinar, Dr. Florea will...

Show all topics (10)

AVATAR Improves transfection efficiency with high viability

May 9, 2018

Now you don’t have to choose between transfection efficiency or viability, you can have both. Use AVATAR with electroporation, chemical transfection, or lipofection workflows to get higher transfection efficiencies with high cell viability in patient-derived cells. AVATAR lets you squeeze and stress your cells for improved transfection, followed by healthy recovery and cell growth using two key microenvironment conditions:  atmospheric pressure and O2 along with CO2 and temperature. AVATAR streamlines and optimizes the entire transfection process. Precise microenvironments along with custom reagents for before, during and post-transfection support your cells of interest and improve DNA and RNA transfection efficiency.

-Cell preconditioning: Primary cells undergo a ‘preconditioning’ and expansion step prior to transfection using a specific AVATAR setting for the cell type. This moves cells into a growth phase over a 48 to 72-hour period followed by a transfection or reprogramming step.

-Cell recovery: After transfection, cells are cultured using another AVATAR setting for approximately 24 hours to improve cell viability and transfection efficiency.

-Cell expansion: Another AVATAR setting is used for rapid expansion and to enrich for transfected cells.

 

Read more!

Application
Product news

Brand profile

Xcell Biosciences

The company provides a revolutionary cell culturing platform that replicates the physiological environment of native tissues to deliver reproducible and biologically relevant molecular analysis.

Related products

The Avatar system can mimic the oxygen and pressure levels of human tissues

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey