Discover

Back to filter

Related topics

Recent publication of in-vivo two-photon intravital imaging study targeting mouse kidney

IVIM Technology

May 22, 2023

In vivo longitudinal 920 nm two-photon intravital kidney imaging of a dynamic 2,8-DHA crystal
formation and
...

Webinar: Multimodal tissue imaging and machine learning to advance precision medicine

RareCyte

May 17, 2023

Join us for this webinar to learn how the Orion spatial biology imaging platform was used to identify prognostic...

Mantis ROI calculator

Formulatrix

May 5, 2023

Decision to improve the output in a lab is always taken based on knowledge and workflow needs. But final decision to...

Real-Time and Quantitative Analysis of Macrophage Phagocytosis with RTCA eSight

Agilent technologies

May 4, 2023

The eSight is currently the only instrument that interrogates cell health and behavior using cellular
impedance
...

Revolutionize your Flow Cytometry and Sorting workflow with Cytek Biosciences

Cytek Biosciences

May 3, 2023

Using full spectrum flow cytometry, Cytek systems ( RUO and CE-IVD) detect the entire fluorochrome emission, allowing...

Thermal Shift Assay using SYPRO Orange to Detect Protein Melting Temperatures

Analytik Jena

May 2, 2023

The thermal shift assay is based on temperature-induced denaturation and can be monitored using SYPRO Orange. This...

A deep learning and Monte Carlo based framework for bioluminescence imaging center Maastro

Precision X-Ray

Apr 20, 2023

"In this paper,we developed a framework using deep learning for bioluminescence-based targeting for GBM animal...

Gentle sorting of microbial cells and sub-micron particles using WOLF sorter

NanoCellect

Apr 18, 2023

While most modern applications of flow cytometry may focus on cells of eukaryotic origin, the first flow analyzers were...

InAlyzer to evaluate G6PD activity in relation with frailty

MEDIKORS

Mar 28, 2023

InAlyzer is body densitometry instrument for lab animals, equipped with 2 X-Ray sources and able to provide valuable...

Cardiomyocyte isolation using Cellenion’s cellenONE instrument

Cellenion

Mar 24, 2023

The cellenONE platform is ideal for the isolation of fragile and heterogeneous cell size populations, such as...

Show all topics (10)

Bronchoscopic confocal fluorescence microscopy for in vivo assessment of free-breathing mice

May 3, 2018

Respiratory diseases, such as pulmonary infections, are an important cause of morbidity and mortality worldwide. Preclinical studies often require invasive techniques to evaluate the extent of infection. Fibered confocal fluorescence microscopy (FCFM) is an emerging optical imaging technique that allows for real-time detection of fluorescently labeled cells within live animals, thereby bridging the gap between in vivo whole-body imaging methods and traditional histological examinations. Previously, the use of FCFM in preclinical lung research was limited to endpoint observations due to the invasive procedures required to access lungs. Here, we introduce a bronchoscopic FCFM approach that enabled in vivo visualization and morphological characterisation of fungal cells within lungs of mice suffering from pulmonary Aspergillus or Cryptococcus infections. The minimally invasive character of this approach allowed longitudinal monitoring of infection in free-breathing animals, thereby providing both visual and quantitative information on infection progression. Both the sensitivity and specificity of this technique were high during advanced stages of infection, allowing clear distinction between infected and non-infected animals. In conclusion, our study demonstrates the potential of this novel bronchoscopic FCFM approach to study pulmonary diseases, which can lead to novel insights in disease pathogenesis by allowing longitudinal in vivo microscopic examinations of the lungs.

Do you want to know more? Read this interesting article!

Scientific paper
Application

Brand profile

Mauna Kea Technologies

Mauna Kea Technologies manufactures Cellvizio Lab, confocal probe based imaging system, providing cellular-level images with minimal invasiveness for longitudinal studies.

Related products

Cellvizio Lab is a single band fiber based microscope for observing in real time at cellular scale

show detail

Cellvizio, a fiber based microscope for observing in real time at cellular scale, features simultaneous, co-registered dual band excitation and detection

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey