Discover

Back to filter

Related topics

Cellular stress induces erythrocyte assembly and promotes microangiopathy

Fluxion Biosciences

Dec 19, 2018

Microangiopathy with subsequent organ damage represents a major complication in several diseases. The mechanisms...

The need for ultra high field magnetic resonance imaging

Bruker Biospin

Dec 17, 2018

MRI  is a noninvasive imaging technology that is known for its superior soft tissue contrast and ability to provide...

NovoCyte Quanteon webinar: In-depth cell Proliferation through new advances in flow cytometry

Acea Biosciences

Dec 11, 2018

Cell proliferation is one of the most fundamental biological processes in biology. Many of the traditional assays to...

Webinar: early detection of metabolic remodeling in pressure overload left ventricle Hypertrophy

Bruker Biospin

Nov 28, 2018

Do you already heard the hypothesis that metabolic changes in the heart may precede and even trigger structural and...

IncuCyte neuronal activity assay

Sartorius

Nov 13, 2018

The generation and maturation of neuronal networks are necessary characteristics of neurogenesis, providing...

The most advanced software for preclinical imaging research

Bruker Biospin

Nov 7, 2018

Modern imaging laboratories hosting PET/MR instruments profit from the fully integrated common imaging platform...

COY - personalized hypoxic chambers

COY laboratory products

Oct 18, 2018

When your research requires consistent atmospheric control to maintain hypoxia, normoxia, or hyperoxia, you can count...

Red blood cell-hitchhiking boosts delivery of nanocarriers to chosen organs

Fluxion Biosciences

Oct 12, 2018

Drug delivery by nanocarriers (NCs) has long been stymied by dominant liver uptake and limited target organ deposition,...

Coy anaerobic chambers #1 choice in microbiology

COY laboratory products

Oct 5, 2018

Used for over 40 years in anaerobic microbiology research, COY chambers are also critical to many other research areas...

Adenovirus type B detection using the xxpress qPCR thermal cycler

BJS Biotechnologies

Sep 17, 2018

The ultrafast xxpress qPCR thermal cycler through performing standard dilution series and multiplex qPCR experiments is...

Show all topics (10)

Bronchoscopic confocal fluorescence microscopy for in vivo assessment of free-breathing mice

May 3, 2018

Respiratory diseases, such as pulmonary infections, are an important cause of morbidity and mortality worldwide. Preclinical studies often require invasive techniques to evaluate the extent of infection. Fibered confocal fluorescence microscopy (FCFM) is an emerging optical imaging technique that allows for real-time detection of fluorescently labeled cells within live animals, thereby bridging the gap between in vivo whole-body imaging methods and traditional histological examinations. Previously, the use of FCFM in preclinical lung research was limited to endpoint observations due to the invasive procedures required to access lungs. Here, we introduce a bronchoscopic FCFM approach that enabled in vivo visualization and morphological characterisation of fungal cells within lungs of mice suffering from pulmonary Aspergillus or Cryptococcus infections. The minimally invasive character of this approach allowed longitudinal monitoring of infection in free-breathing animals, thereby providing both visual and quantitative information on infection progression. Both the sensitivity and specificity of this technique were high during advanced stages of infection, allowing clear distinction between infected and non-infected animals. In conclusion, our study demonstrates the potential of this novel bronchoscopic FCFM approach to study pulmonary diseases, which can lead to novel insights in disease pathogenesis by allowing longitudinal in vivo microscopic examinations of the lungs.

Do you want to know more? Read this interesting article!

Scientific paper
Application

Related technologies: Probe based confocal microscopy

Brand profile

Mauna Kea Technologies

Mauna Kea Technologies manufactures Cellvizio Lab, confocal probe based imaging system, providing cellular-level images with minimal invasiveness for longitudinal studies.

Related products

Cellvizio Lab is a single band fiber based microscope for observing in real time at cellular scale

show detail

Cellvizio, a fiber based microscope for observing in real time at cellular scale, features simultaneous, co-registered dual band excitation and detection

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey