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Observe the movement of live cells that make up living tissue in vivo!

IVIM Technology

Apr 12, 2021

IntraVital Microscopy is a technique that enables you to directly observe the movement of live cells that make up...

Single-cell DNA Analysis of a Novel Acute Erythroid Leukemia CRISPR Induced Mouse Model

Mission Bio

Apr 12, 2021

Single-cell DNA Analysis of a Novel Acute Erythroid Leukemia (AEL) CRISPR Induced Mouse Model.
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SARS-CoV-2 detection workflow in wastewater- Analytik Jena and project in Japan

Analytik Jena

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In 2020 Analytik Jenas real-time PCR thermal cyclers of the qTOWER³ series even supported one of the first SARS-CoV-2...

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Radio-active sources have been used routinely for the preconditioning in humanized mouse...

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Fluxion Biosciences

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Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes...

Do you have our CellRad benchtop irradiator? It’s the only one on the market!

Precision X-Ray

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The CellRad is a smaller, simpler, safer, and more cost effective alternative to...

Dosimetric characterization of an X-ray irradiator for use with cells

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This study aims to characterize an X-ray irradiator for use with cells using its internal...

Early immune response in mice immunized with inactivated vaccine against SARS-CoV-2

Cytek Biosciences

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The development of a vaccine against COVID-19 is a hot topic for many research...

Cellular stress induces erythrocyte assembly and promotes microangiopathy

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Microangiopathy with subsequent organ damage represents a major complication in several diseases. The mechanisms...

The need for ultra high field magnetic resonance imaging

Bruker Biospin

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MRI  is a noninvasive imaging technology that is known for its superior soft tissue contrast and ability to provide...

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Evaluation of signaling and drug response of cancer cells culture in 2D vs 3D

Jan 25, 2017

Spheroids showed relative lower activities in the AKT, mammalian target of rapamycin (mTOR) and S6K (also known as RPS6KB1) signaling pathway compared to cells cultured in two dimensions. We identified spatial alterations in signaling, as the level of phosphorylated RPS6 decreased from the spheroid surface towards the center, which closely coordinated with the tumor areas around vessels in vivo. These 3D models displayed augmented antitumor responses to AKT–mTOR–S6K or mitogen-activated protein kinase (MAPK) pathway inhibition compared to those in 2D models. Inhibition of AKT–mTOR–S6K resulted in elevated ERK phosphorylation in 2D culture, whereas under these conditions, ERK signaling was reduced in spheroids. Inhibition of MEK1 (also known as MAP2K1) led to decreased AKT–mTOR–S6K signaling in 3D but not in 2D culture. These data indicate a distinct rewiring of signaling in 3D culture and during treatment. Detached tumor-cell clusters in vessels, in addition to circulating single tumor cells, play a putative role in metastasis in human cancers. Hence, the understanding of signaling in spheroids and the responses in the 3D models upon drug treatment might be beneficial for anti-cancer therapies.

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TissueGnostics

TissueGnostics develops systems for automated identification and functional characterization of single cells in tissue sections, cultured and smeared cells as well as TMA. This includes automated image acquisition and image processing for immunohistochemistry and multi-chanel immunofluorescence.

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Inverted fluorescence and brightfield system for the scanning and analysis of slides, cytospins, smears and Tissue Microarrays.

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