Discover

Back to filter

Related topics

Automating DNA Extraction for High-Throughput Bacterial Plasmid Purification with CyBi®-SELMA

Analytik Jena

Sep 19, 2022

The Need to Increase Plasmid Isolation Throughput, Challenges with Scaling up Plasmid DNA Extraction, Automating the...

Transcriptome-Informed Approach to Drug Discovery

HTG Molecular Diagnostics

Jul 12, 2022

The HTG EdgeSeq system is a powerful molecular profiling tool for biopharmaceutical companies, academic research...

Semi-automated genomic DNA extractionfrom Plant with CyBi®-SELMA

Analytik Jena

Jul 12, 2022

Plant genomic DNA extraction with CyBio SELMA, Semi-Automated Pipette with minimal benchtop footprint

Outstanding post-sort sample recovery on the WOLF Cell Sorter

NanoCellect

Jun 14, 2022

The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting...

Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters

MOLECUBES

Jun 3, 2022

Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters, whereas hydroxychloroquine...

Quantitative 3D Optical Imaging for Lago

Spectral Instruments Imaging

Feb 21, 2022

Spectral Instrument Imaging just announced a new, strategic partnership with InVivo Analytics, which enables...

Extensive assessment of Cytokine production on the NovoCyte Penteon flow cytometer

Agilent technologies

Nov 29, 2021

Cytokines are small molecules essential for immune cell response to activation by pathogens, autoimmunity, or...

How to Improve Your Viral Nucleic Acid Extraction Processes

Analytik Jena

Sep 6, 2021

High-Throughput Extraction with CyBio FeliX

Comparison of gamma and x-ray irradiation results

Precision X-Ray

Aug 12, 2021

Biological effects of Cs-137 and X-ray irradiators are comparable with regards to...

Hypoxia further exacerbates myopathy in broilers via alteration of satellite cell fate

COY laboratory products

Aug 2, 2021

Woody breast (WB) condition has created a variety of challenges for the global poultry...

Show all topics (10)

Evaluation of signaling and drug response of cancer cells culture in 2D vs 3D

Jan 25, 2017

Spheroids showed relative lower activities in the AKT, mammalian target of rapamycin (mTOR) and S6K (also known as RPS6KB1) signaling pathway compared to cells cultured in two dimensions. We identified spatial alterations in signaling, as the level of phosphorylated RPS6 decreased from the spheroid surface towards the center, which closely coordinated with the tumor areas around vessels in vivo. These 3D models displayed augmented antitumor responses to AKT–mTOR–S6K or mitogen-activated protein kinase (MAPK) pathway inhibition compared to those in 2D models. Inhibition of AKT–mTOR–S6K resulted in elevated ERK phosphorylation in 2D culture, whereas under these conditions, ERK signaling was reduced in spheroids. Inhibition of MEK1 (also known as MAP2K1) led to decreased AKT–mTOR–S6K signaling in 3D but not in 2D culture. These data indicate a distinct rewiring of signaling in 3D culture and during treatment. Detached tumor-cell clusters in vessels, in addition to circulating single tumor cells, play a putative role in metastasis in human cancers. Hence, the understanding of signaling in spheroids and the responses in the 3D models upon drug treatment might be beneficial for anti-cancer therapies.

Read more

Scientific paper

Brand profile

TissueGnostics

TissueGnostics develops systems for automated identification and functional characterization of single cells in tissue sections, cultured and smeared cells as well as TMA. This includes automated image acquisition and image processing for immunohistochemistry and multi-chanel immunofluorescence.

Related products

Inverted fluorescence and brightfield system for the scanning and analysis of slides, cytospins, smears and Tissue Microarrays.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey