Discover

Back to filter

Related topics

Pretargeted delivery of PI3K/mTOR small-molecule inhibitor–loaded nanoparticles

Spectral Instruments Imaging

Jun 23, 2021

Overactivation of the PI3K/mTOR signaling has been identified in non-Hodgkin’s lymphoma....

Elevated NSD3 histone methylation activity drives squamous cell lung cancer

Spectral Instruments Imaging

Jun 8, 2021

This work identifies NSD3 as a principal 8p11-12 amplicon-associated oncogenic driver in...

Single-cell DNA Analysis of a Novel Acute Erythroid Leukemia CRISPR Induced Mouse Model

Mission Bio

May 9, 2021

Assessment of precise genetic edits. Analysis of the clonal structure & co-mutations of AEL. Use of CRISPR and...

SARS-CoV-2 detection workflow in wastewater - Analytik Jena and project in Japan

Analytik Jena

May 8, 2021

In 2020 Analytik Jena real-time PCR thermal cyclers of the qTOWER³ series even supported one of the first SARS-CoV-2...

Cytek Spectral cytometers : taking science one step further with 200+ Publications

Cytek Biosciences

Apr 26, 2021

Cytek Aurora is helping researchers around the world take their science further. See how...

Observe the movement of live cells that make up living tissue in vivo!

IVIM Technology

Apr 12, 2021

IntraVital Microscopy is a technique that enables you to directly observe the movement of live cells that make up...

Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice

Precision X-Ray

Dec 29, 2020

Radio-active sources have been used routinely for the preconditioning in humanized mouse...

Advances in Leukemia research using shear flow and Bioflux system

Fluxion Biosciences

Dec 10, 2020

Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes...

Do you have our CellRad benchtop irradiator? It’s the only one on the market!

Precision X-Ray

Dec 9, 2020

The CellRad is a smaller, simpler, safer, and more cost effective alternative to...

Dosimetric characterization of an X-ray irradiator for use with cells

Precision X-Ray

Dec 4, 2020

This study aims to characterize an X-ray irradiator for use with cells using its internal...

Show all topics (10)

Foot-and-mouth deseases virus research using IncuCyte ZOOM

Apr 21, 2016

Probing the molecular interactions within the foot-and-mouth disease virus (FMDV) RNA replication complex has been restricted in part by the lack of suitable reagents. Random insertional mutagenesis has proven an excellent method to reveal domains of proteins essential for virus replication as well as locations that can tolerate small genetic insertions. Such insertion sites can subsequently be adapted by the incorporation of commonly used epitope tags, facilitating their detection with commercially available reagents. In this study, we used random transposon-mediated mutagenesis to produce a library of 15 nt insertions in the FMDV nonstructural polyprotein. Using a replicon-based assay, we isolated multiple replication-competent as well as replication-defective insertions. We adapted the replication-competent insertion sites for the successful incorporation of epitope tags within FMDV non-structural proteins for use in a variety of downstream assays. Additionally, we showed that replication of some of the replication-defective insertion mutants could be rescued by co-transfection of a ‘helper’ replicon, demonstrating a novel use of random mutagenesis to identify intergenomic transcomplementation. Both the epitope tags and replication-defective insertions identified here will be valuable tools for probing interactions within picornavirus replication complexes.

Read more

Scientific paper

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey