Optical tweezers, Confocal microscopy, STED nanoscopy, Acoustic force spectroscopy for single-molecule biophysics.
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High-throughput observation of molecular motor activity & dynamics
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The cytoskeleton and its many molecular motors are major players in a variety of cellular functions. The interconnected networks of cytoskeletal filaments act as tracks directing molecular motor transport and provide structural support and organization to cells. With optical tweezers, we are capable of resolving sub-nanometer displacements and applying pico-Newton forces on single proteins. This force spectroscopy approach has proven extremely useful in elucidating the mechanics and kinetics of molecular motors involved in intracellular transport.
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