Coy Labs produces Glove Boxes, Anaerobic Chambers, and controlled atmosphere glove boxes.
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Diagnosis of Acute Cellular Rejection Using Probe-Based Confocal Laser Endomicroscopy in Lung
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Differentation displacement in acute myeloid leukemia through enhancer activation
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Blast cell differentation is inhibitited by pharmacological targeting of lysine specific demethylase 1 (LSD1). In this...
Dynamic PET imaging and special focus in cardiac kinetic analysis
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Hypoxia activates constitutive Luciferase reporter constructs
Jul 13, 2017
Studies in our laboratory sought to examine the hypoxic regulation of HIF activity in placental trophoblast cells, through the use of dual-reporter luciferase assays. Our study demonstrates that hypoxic conditions cause a significant increase in the level of constitutive luciferase reporter activity. We also show that this induction is not a cell-type or species-specific phenomenon and provides an alternative method for normalizing transfection efficiency in luciferase assays under hypoxic conditions. Our results suggest that in studies dealing with hypoxic conditions, caution should be used when interpreting measurements of transcriptional activity by traditional dual-reporter assays.
Related technologies: Hypoxia and O2 controlled environment isolators | Anaerobic boxes
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