Coy Labs produces Glove Boxes, Anaerobic Chambers, and controlled atmosphere glove boxes.
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Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice
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Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes...
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Dosimetric characterization of an X-ray irradiator for use with cells
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Early immune response in mice immunized with inactivated vaccine against SARS-CoV-2
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Cellular stress induces erythrocyte assembly and promotes microangiopathy
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Microangiopathy with subsequent organ damage represents a major complication in several diseases. The mechanisms...
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The most advanced software for preclinical imaging research
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Red blood cell-hitchhiking boosts delivery of nanocarriers to chosen organs
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Adenovirus type B detection using the xxpress qPCR thermal cycler
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Hypoxia activates constitutive Luciferase reporter constructs
Jul 13, 2017
Studies in our laboratory sought to examine the hypoxic regulation of HIF activity in placental trophoblast cells, through the use of dual-reporter luciferase assays. Our study demonstrates that hypoxic conditions cause a significant increase in the level of constitutive luciferase reporter activity. We also show that this induction is not a cell-type or species-specific phenomenon and provides an alternative method for normalizing transfection efficiency in luciferase assays under hypoxic conditions. Our results suggest that in studies dealing with hypoxic conditions, caution should be used when interpreting measurements of transcriptional activity by traditional dual-reporter assays.
Related technologies: Hypoxia and O2 controlled environment isolators | Anaerobic boxes
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