Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.
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Recent publication of in-vivo two-photon intravital imaging study targeting mouse kidney
May 22, 2023
In vivo longitudinal 920 nm two-photon intravital kidney imaging of a dynamic 2,8-DHA crystal
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Webinar: Multimodal tissue imaging and machine learning to advance precision medicine
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Join us for this webinar to learn how the Orion spatial biology imaging platform was used to identify prognostic...
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Decision to improve the output in a lab is always taken based on knowledge and workflow needs. But final decision to...
Real-Time and Quantitative Analysis of Macrophage Phagocytosis with RTCA eSight
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The eSight is currently the only instrument that interrogates cell health and behavior using cellular
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Revolutionize your Flow Cytometry and Sorting workflow with Cytek Biosciences
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Using full spectrum flow cytometry, Cytek systems ( RUO and CE-IVD) detect the entire fluorochrome emission, allowing...
Thermal Shift Assay using SYPRO Orange to Detect Protein Melting Temperatures
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The thermal shift assay is based on temperature-induced denaturation and can be monitored using SYPRO Orange. This...
A deep learning and Monte Carlo based framework for bioluminescence imaging center Maastro
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"In this paper,we developed a framework using deep learning for bioluminescence-based targeting for GBM animal...
Gentle sorting of microbial cells and sub-micron particles using WOLF sorter
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While most modern applications of flow cytometry may focus on cells of eukaryotic origin, the first flow analyzers were...
InAlyzer to evaluate G6PD activity in relation with frailty
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Cardiomyocyte isolation using Cellenion’s cellenONE instrument
Mar 24, 2023
The cellenONE platform is ideal for the isolation of fragile and heterogeneous cell size populations, such as...
Jul 9, 2018
Tumours are composed of cancer cells and different types of stromal cells. Stromal cells help to create the special microenvironment which surrounds a tumour and therefore they affect proliferation, invasion and metastasis of tumours. From the stromal cells, CAFs are the most abundant. Even though they exhibit a homogenous morphology, phenotypically and functionally the CAFs are very heterogeneous. This makes in vivo and in Vitro studies more difficult with an indication that the cells are coming from various parts of the body. In the study, two primary CAF cultures from lung adenocarcinoma were transduced with human telomerase reverse transcriptase mutant. They were imaged on Incucyte and the number of successful single-cell origin colonies was counted. Then the expression of genes associated with tumour progression pathways was evaluated by qRT-PCR. As the results of the observation part, the researchers observed changes in lifespan and proliferation depending on the gene that was transduced in the cells. They also evaluated the mRNA levels of seven growth-factor related genes and three genes which were previously associated with pathways promoting tumour growth to study these phenomena further. This was done on fourteen clones and it was showed that the level of mRNA expression differs drastically between them. The study helps to create a deeper understanding of factor which functionally influences tumours and their progression. The method that the researchers developed can be further used to study clones and observe their effect on the cancerous microenvironment.
Related technologies: BioImaging
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Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.
More info at:
www.essenbioscience.com/