Discover

Back to filter

Related topics

Intoducing spheroOne: Automated sorting and isolation of single spheroids and organoids

Cellenion

Jun 21, 2022

spheroONE is an innovative single large-particle sorter and dispenser which revolutionizes 3D cellular models handling....

Outstanding post-sort sample recovery on the WOLF Cell Sorter

NanoCellect

Jun 14, 2022

The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting...

Incucyte AI module: Advanced Label-Free Classification Analysis

Sartorius

Jun 6, 2022

Monitor adherent cell morphology changes objectively to determine live/dead cell counts or classify cells based on...

Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters

MOLECUBES

Jun 3, 2022

Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters, whereas hydroxychloroquine...

Intoducing cellenONE: The revolutionary Single Cell isolator

Cellenion

May 25, 2022

A revolutionary platform based on sciDROP PICO precision dispensing technology and coupled with advanced image...

Advances in Leukemia research using shear flow and Bioflux system

Fluxion Biosciences

May 24, 2022

Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes create disruptions in the immune...

Why use Cytek Aurora?

Cytek Biosciences

May 4, 2022

A prodigy incorporating a unique combination of patent-pending innovative technologies that takes flow cytometry to the...

Introducing Celloger Mini Plus: Automated and compact Live cell imaging inside your incubator

Curiosis

Apr 11, 2022

We are happy to introduce new Celloger Mini Plus, automated live cell imaging system! Click the video below to find out...

CQ1 live-cell confocal system: revealing the pathophysiology of COVID-19 infection

Yokogawa

Mar 21, 2022

Yokogawa decided to support the world’s fight against COVID-19, scientifically cooperating with research facilities...

In-vivo webinar: a versatile use of SPECT/CT in the development of theranostic tracers

MOLECUBES

Mar 9, 2022

Combining (preclinical) imaging and targeted therapy, is the basis of theranostics. In this webinar, Dr. Florea will...

Show all topics (10)

Introducing IncuCyte antibody Internalization assays

Jan 22, 2018

Antigen-mediated antibody internalization plays an important role in several antibody-based therapeutics. There is a continued growing body of interest in the application of antibodies as therapeutic agents for delivery of treatments to sites or cells of interest with promise of increased efficacy and reduced side effect potential.The significance and impact of therapeutic antibodies as effective treatments for disease span across numerous areas such as autoimmunity, cancer and inflammation, cardiovascular and infectious diseases, and many other disorders.The evolving therapeutic impact of antibody internalization on the antibody discovery field and targeted-mediated internalization includes the delivery of highly toxic drugs to cancer cells via antibody drug conjugates (ADCs), removal or degradation of surface receptors from cancer cells (i.e. EGFR), and antibody immunotherapies used to identify tumor cells for immune cell killing (i.e. ADCC or ADCP). Additionally, measuring and optimizing functional responses to antibodies, such as antibody clearance is of importance. Pinocytosis, which is one of the main elimination routes of antibodies, requires antibody optimization for qualitative pharmacokinetic measurements during antibody development. As each approach requires a series of antibody features, for example, to enable maintenance on the cell surface for identification of tumor cells, or for rapid internalization when delivering ADC’s, it is important to understand the uptake profile and clearance of antibody candidates for optimal antibody engineering and internalization characteristics.

Current approaches are limited by requiring labor-intensive labeling of each antibody, washing steps, and/or single-time point analysis. This can lead to unnecessary and lengthy processes, loss of cells, or missed and incorrect measurements due to limitations of single time-point analysis. Therefore, throughout the entire antibody screening and functional characterization process, there is a strong need to have an efficient, affordable method to fully and rapidly quantify antibody internalization using real time live-cell analysis.Essen Bioscience is proud to offer a new, novel solution that is compatible with both human and mouse test antibodies (utilizing IncuCyte Human and Mouse IgG1 FabFluor red antibody labeling reagent) for rapid, kinetic, high-throughput analysis of antibody internalization.

Read more

Scientific paper
Application
Product news
Video

Related technologies: BioImaging

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

Related products

High throughput, real-time and automated measurements of live-cell activity, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey