Discover

Back to filter

Related topics

Introducing Celloger Mini: Automated and compact Live cell imaging inside your incubator

Curiosis

Jul 23, 2021

Celloger Mini is an automated live cell imaging system based on bright-field microscopy...

High quality Bioluminescence and X-Ray analysis in lung squamous cell carcinoma

Spectral Instruments Imaging

Jul 21, 2021

Spectral Instruments Imaging provides unrivaled sensitivity for bioluminescence,...

Pretargeted delivery of PI3K/mTOR small-molecule inhibitor–loaded nanoparticles

Spectral Instruments Imaging

Jul 3, 2021

Overactivation of the PI3K/mTOR signaling has been identified in non-Hodgkin’s lymphoma....

Quantitative two-photon microscopy imaging analysis of human skin

IVIM Technology

Jul 1, 2021

Transdermal skin delivery is a method to transport various topical formulations to a deeper skin layer non-invasively....

Elevated NSD3 histone methylation activity drives squamous cell lung cancer

Spectral Instruments Imaging

Jun 8, 2021

This work identifies NSD3 as a principal 8p11-12 amplicon-associated oncogenic driver in...

A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic

Tonbo Biosciences

May 21, 2021

Tonbo Biosciences flow cytometry reagents & antibodies are manufactured with the highest...

Single-cell DNA Analysis of a Novel Acute Erythroid Leukemia CRISPR Induced Mouse Model

Mission Bio

May 9, 2021

Assessment of precise genetic edits. Analysis of the clonal structure & co-mutations of AEL. Use of CRISPR and...

SARS-CoV-2 detection workflow in wastewater - Analytik Jena and project in Japan

Analytik Jena

May 8, 2021

In 2020 Analytik Jena real-time PCR thermal cyclers of the qTOWER³ series even supported one of the first SARS-CoV-2...

IVM-MS2: the most compact IntraVital Two-Photon microscope in the world

IVIM Technology

Apr 28, 2021

IVIM Technology, Inc. has released the most compact all-in-one intravital two-photon microscope. IVM-MS2 is the world's...

Cytek Spectral cytometers : taking science one step further with 200+ Publications

Cytek Biosciences

Apr 26, 2021

Cytek Aurora is helping researchers around the world take their science further. See how...

Show all topics (10)

Label-free, real-time live-cell assays for spheroids

Aug 3, 2017

Tumor spheroids exhibit more relevant morphology and increased cell survival compared to 2D cultures and have a hypoxic core. Current methods for assessing the growth and shrinkage of tumor spheroids are limited by one or more of the following: (1) assay workflows are time-consuming, expensive or laborious, (2) a requirement to label the cells (e.g. a fluorescent probe) which may perturb the biology and may not be amenable to primary tissue, (3) single time-point readouts that do not report the full timecourse, (4) indirect readouts (e.g. ATP) that may overlook valuable morphological insight and/or mis-report cell growth.
In this application note we describe methods and validation data for miniaturized (96/384-well) live-cell tumor spheroid assays that are based on non-invasive bright-field image analysis performed with the IncuCyte S3 Spheroid software module. Tumor spheroids are formed in ultra-low attachment (ULA) plates and monitored for size and morphology for up to 2 weeks. These assays are flexible, simple to run and provide automated and direct measures of tumor size in real-time.

Read more

 

Scientific paper
Product news

Related technologies: BioImaging

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

Related products

High throughput, real-time and automated measurements of live-cell activity, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey