Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.
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Intoducing cellenONE: The revolutionary Single Cell isolator
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Introducing Celloger Mini Plus: Automated and compact Live cell imaging inside your incubator
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CQ1 live-cell confocal system: revealing the pathophysiology of COVID-19 infection
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Yokogawa decided to support the world’s fight against COVID-19, scientifically cooperating with research facilities...
Introducing IsoPlexis Single-Cell Natural Killer Panel
Mar 4, 2022
Because of their ability to kill tumour cells, NK cells are an attractive target in cancer immunotherapy, therapeutic...
Enhanced small particle detection on CytekAurora and Northern Lights
Mar 2, 2022
Aurora system with ESP option, can fully resolve particles around 70nm and from the 80nm PS bead. The new violet laser...
Quantitative 3D Optical Imaging for Lago
Feb 21, 2022
Spectral Instrument Imaging just announced a new, strategic partnership with InVivo Analytics, which enables...
Aurora CS spectral sorter - 28 Color in-depth purity sorting
Jan 28, 2022
To demonstrate the Cytek Aurora CS’s capabilities made possible by its advanced electronics design, a 28-color human...
Download today your IsoPlexis e-book: understanding immune Cell Function In Cancer
Dec 1, 2021
Understanding complex immune-cancer interactions is critical to identifying therapeutic targets, devising novel avenues...
Extensive assessment of Cytokine production on the NovoCyte Penteon flow cytometer
Nov 29, 2021
Cytokines are small molecules essential for immune cell response to activation by pathogens, autoimmunity, or...
Label-free, real-time live-cell assays for spheroids
Aug 3, 2017
Tumor spheroids exhibit more relevant morphology and increased cell survival compared to 2D cultures and have a hypoxic core. Current methods for assessing the growth and shrinkage of tumor spheroids are limited by one or more of the following: (1) assay workflows are time-consuming, expensive or laborious, (2) a requirement to label the cells (e.g. a fluorescent probe) which may perturb the biology and may not be amenable to primary tissue, (3) single time-point readouts that do not report the full timecourse, (4) indirect readouts (e.g. ATP) that may overlook valuable morphological insight and/or mis-report cell growth.
In this application note we describe methods and validation data for miniaturized (96/384-well) live-cell tumor spheroid assays that are based on non-invasive bright-field image analysis performed with the IncuCyte S3 Spheroid software module. Tumor spheroids are formed in ultra-low attachment (ULA) plates and monitored for size and morphology for up to 2 weeks. These assays are flexible, simple to run and provide automated and direct measures of tumor size in real-time.
Related technologies: BioImaging
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