Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.
Discover
Back to filter
Show all topics (10)
Related topics
Automating DNA Extraction for High-Throughput Bacterial Plasmid Purification with CyBi®-SELMA
Sep 19, 2022
The Need to Increase Plasmid Isolation Throughput, Challenges with Scaling up Plasmid DNA Extraction, Automating the...
Introducing RareCyte Orion, integrating Spatial Biology with Multiplex Imaging
Sep 13, 2022
Orion, the highly multiplexed fluorescent imaging system, empower investigators to unlock spatial context for key...
xCELLigence eSight - Imaging & Impedance in real-time inside your incubator
Aug 22, 2022
The xCELLigence RTCA eSight enables comprehensive insight into cell health, cell behavior, cell function and cell...
Standardize tumor sample preparation and reduce background with Curiox laminar wash system
Aug 10, 2022
Laminar Wash technology offers a gentle alternative that can improve yield and data quality, particularly of rare cell...
Developing a 43 color panel on Cytek Aurora using Laminar Wash for sample prep
Aug 2, 2022
Successfully executing a 43 color panel is a technically demanding task. One that is made more difficult when there are...
Transcriptome-Informed Approach to Drug Discovery
Jul 12, 2022
The HTG EdgeSeq system is a powerful molecular profiling tool for biopharmaceutical companies, academic research...
Semi-automated genomic DNA extractionfrom Plant with CyBi®-SELMA
Jul 12, 2022
Plant genomic DNA extraction with CyBio SELMA, Semi-Automated Pipette with minimal benchtop footprint
New Center of Excellence, located at the Josep Carreras Leukaemia Research Institute
Jul 12, 2022
Mission Bio is a life sciences company that accelerates discoveries and cures for a wide range of diseases by equipping...
Intoducing spheroOne: Automated sorting and isolation of single spheroids and organoids
Jun 21, 2022
spheroONE is an innovative single large-particle sorter and dispenser which revolutionizes 3D cellular models handling....
Outstanding post-sort sample recovery on the WOLF Cell Sorter
Jun 14, 2022
The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting...
Label-free, real-time live-cell assays for spheroids
Aug 3, 2017
Tumor spheroids exhibit more relevant morphology and increased cell survival compared to 2D cultures and have a hypoxic core. Current methods for assessing the growth and shrinkage of tumor spheroids are limited by one or more of the following: (1) assay workflows are time-consuming, expensive or laborious, (2) a requirement to label the cells (e.g. a fluorescent probe) which may perturb the biology and may not be amenable to primary tissue, (3) single time-point readouts that do not report the full timecourse, (4) indirect readouts (e.g. ATP) that may overlook valuable morphological insight and/or mis-report cell growth.
In this application note we describe methods and validation data for miniaturized (96/384-well) live-cell tumor spheroid assays that are based on non-invasive bright-field image analysis performed with the IncuCyte S3 Spheroid software module. Tumor spheroids are formed in ultra-low attachment (ULA) plates and monitored for size and morphology for up to 2 weeks. These assays are flexible, simple to run and provide automated and direct measures of tumor size in real-time.
Related technologies: BioImaging
Brand profile
