Discover

Back to filter

Related topics

Curiox webinar - how to retain more cells for Single Cell Sequencing and proteogenomics ?

Curiox

Feb 15, 2021

Curiox Laminar Wash technology has been added as part of the official BioLegend TotalSeq...

Cytek Aurora in Action : how to easily resolve highly overlapping dyes ?

Cytek Biosciences

Feb 2, 2021

Learn from Jeff Nelson, a manager of a cytometry shared resource laboratory, about the...

Webinar: A simple Migration/Invasion workflow using IncuCyte Live-cell analysis system

Sartorius

Jan 27, 2021

Cell migration and invasion, or cell motility, play a role in many normal and...

Webinar : meet the Cytek's new cFluor reagents

Cytek Biosciences

Jan 25, 2021

Cytek Biosciences has already redefined what is possible in flow cytometry. The new Cytek...

Introducing Cytek cFluor, 14 Colors Immunoprofiling Kit

Cytek Biosciences

Jan 19, 2021

Cytek cFluor Immunoprofiling Kit allows for the identification of helper T cells,...

Cytek full Spectrum flow Cytometry webinar: Part II – panel design and troubleshooting

Cytek Biosciences

Jan 11, 2021

In this session, we will continue to look at full spectrum flow cytometry and look at considerations for fluorescence...

IncuCyte for in-vitro analysis of SARS-CoV-2 vaccine candidate

Sartorius

Jan 4, 2021

Watch this video to learn how researcher at Humane Genomics are utilizing the Incucyte...

Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice

Precision X-Ray

Dec 29, 2020

Radio-active sources have been used routinely for the preconditioning in humanized mouse...

Coming in spring 2021, meet the Cytek Aurora CS

Cytek Biosciences

Dec 18, 2020

Ever wanted to take your full spectrum panel from your Cytek Aurora or Northern Lights...

Advances in Leukemia research using shear flow and Bioflux system

Fluxion Biosciences

Dec 10, 2020

Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes...

Show all topics (10)

Label-free, real-time live-cell assays for spheroids: IncuCyte bright-field analysis

Feb 12, 2018

Current methods for assessing the growth and shrinkage  of  tumor  spheroids  are  limited  by  one  or  more  of  the  following:  (1)  assay  workflows  that  are  time-consuming,  expensive  or  laborious,  (2)  a  requirement  to  label  the  cells  (e.g.  a  fluorescent  probe)  which  may  perturb  the  biology  and  may  not  be  amenable  to  primary  tissue,  (3)  single  time-point  readouts  that  do  not  report  the  full  timecourse,  (4)  indirect  readouts (e.g. ATP) that may overlook  valuable morphological insight and/or mis-report cell growth.In  this  application  note  we  describe  methods  and  validation  data  for  miniaturized  (96/384-well)  live-cell  tumor  spheroid  assays   that   are   based   on   non-invasive   bright-field   image   analysis  performed  with  the  IncuCyte  S3  Spheroid  software  module.  Tumor  spheroids  are  formed  in  ultra-low  attachment  (ULA) plates and monitored for size and morphology for up to 2  weeks.  These  assays  are  flexible,  simple  to  run  and  provide  automated and direct measures of tumor size in real-time.

Read more

 

Scientific paper
Application
Product news

Related technologies: BioImaging

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

Related products

High throughput, real-time and automated measurements of live-cell activity, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey