Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.
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Download today your IsoPlexis e-book: understanding immune Cell Function In Cancer
Dec 1, 2021
Understanding complex immune-cancer interactions is critical to identifying therapeutic targets, devising novel avenues...
Extensive assessment of Cytokine production on the NovoCyte Penteon flow cytometer
Nov 29, 2021
Cytokines are small molecules essential for immune cell response to activation by pathogens, autoimmunity, or...
IsoPlexis technology: accelerating decisions in immunotherapy with predictive single-cell response
Nov 26, 2021
The IsoLight is a hub for comprehensive functional profiling of each cell type across a large assay menu of single-cell...
Customer Story: Automation at DKMS LIFE SCIENCE LAB
Nov 25, 2021
How DKMS LIFE SCIENCE LAB is Supporting the Fight against Leukemia with New Processes and Automation
Accelerating AML Research Through Single-cell Multi-omics- integrate seamlessly into existing NGS
Nov 25, 2021
Dr. Linde Miles Memorial Sloan Kettering Cancer Center about her research, use of the Tapestri as a single-cell...
The New Standard in Automated Nucleic Acid Extraction- InnuPure C16
Nov 23, 2021
Fully automatic nucleic acid extraction of up to 16 samples, sophisticated kit architecture for easy handling, more...
Discover the advanced Detector Technology in Agilent NovoCyte flow cytometers
Nov 18, 2021
This technical overview describes advancements in detector technology, and highlights examples where optimal signal...
Introducing isoLight system - Automated Cellular Proteomics hub
Nov 16, 2021
The IsoLight is a hub for comprehensive functional profiling of each cell type across a large assay menu of single-cell...
Introducing Cytek NEW 25-Color Immunoprofiling Assay & Kit!
Nov 15, 2021
To unleash the power of the Cytek full spectrum cytometry system, Cytek scientists designed and developed the 25-Color...
WEBINAR: Recent Advancements In Neurodegenerative Biomarkers
Sep 6, 2021
Recent Advancements in Neurodegenerative Biomarkers: Progress Towards a Diagnostic Blood Test for Alzheimer’s Disease
Label-free, real-time live-cell assays for spheroids: IncuCyte bright-field analysis
Feb 12, 2018
Current methods for assessing the growth and shrinkage of tumor spheroids are limited by one or more of the following: (1) assay workflows that are time-consuming, expensive or laborious, (2) a requirement to label the cells (e.g. a fluorescent probe) which may perturb the biology and may not be amenable to primary tissue, (3) single time-point readouts that do not report the full timecourse, (4) indirect readouts (e.g. ATP) that may overlook valuable morphological insight and/or mis-report cell growth.In this application note we describe methods and validation data for miniaturized (96/384-well) live-cell tumor spheroid assays that are based on non-invasive bright-field image analysis performed with the IncuCyte S3 Spheroid software module. Tumor spheroids are formed in ultra-low attachment (ULA) plates and monitored for size and morphology for up to 2 weeks. These assays are flexible, simple to run and provide automated and direct measures of tumor size in real-time.
Related technologies: BioImaging
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