Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.
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Dec 1, 2022
Cytek Cloud is the new digital ecosystem that supports full spectrum flow cytometry research from panel design to data...
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Nov 28, 2022
Still wondering whether to join the shift to full spectrum cytometry? Easily transfer assays from your Cytek Northern...
Demonstrate the value of RareCyte Orion system via web-based Minerva viewer
Nov 25, 2022
The HTA CRC Atlas X dataset contains images and other data being used for construction of an atlas of human colorectal...
Practical aspects of an imaging study: live in vivo scanning
Nov 11, 2022
In this webinar, MOLECUBES will present a live in vivo demonstration, showing the entire workflow from animal...
Download the newest AVATAR data presented at CAR-TCR
Oct 21, 2022
Xcell Bio CSO, James Lim, presented new data showcasing improved potency of AVATAR-expanded CAR T cells and highlighted...
RareCyte Orion system advances Multi-modal digital pathology for colorectal cancer diagnosis
Oct 19, 2022
Precision medicine is critically dependent on better methods for diagnosing and staging disease and predicting drug...
Introducing MARS system : revolutionary solution for your workflow of cell separation and enrichment
Oct 7, 2022
The MARS family of instruments provides a breakthrough solution to complete the workflow of cell separation and...
Understand the Cancer Ecosystem based on single cells analysis
Sep 22, 2022
Mission Bio is a life sciences company that accelerates discoveries and cures for a wide range of diseases by equipping...
Automating DNA Extraction for High-Throughput Bacterial Plasmid Purification with CyBi®-SELMA
Sep 19, 2022
The Need to Increase Plasmid Isolation Throughput, Challenges with Scaling up Plasmid DNA Extraction, Automating the...
Introducing RareCyte Orion, integrating Spatial Biology with Multiplex Imaging
Sep 13, 2022
Orion, the highly multiplexed fluorescent imaging system, empower investigators to unlock spatial context for key...
Label-free, real-time live-cell assays for spheroids: IncuCyte bright-field analysis
Feb 12, 2018
Current methods for assessing the growth and shrinkage of tumor spheroids are limited by one or more of the following: (1) assay workflows that are time-consuming, expensive or laborious, (2) a requirement to label the cells (e.g. a fluorescent probe) which may perturb the biology and may not be amenable to primary tissue, (3) single time-point readouts that do not report the full timecourse, (4) indirect readouts (e.g. ATP) that may overlook valuable morphological insight and/or mis-report cell growth.In this application note we describe methods and validation data for miniaturized (96/384-well) live-cell tumor spheroid assays that are based on non-invasive bright-field image analysis performed with the IncuCyte S3 Spheroid software module. Tumor spheroids are formed in ultra-low attachment (ULA) plates and monitored for size and morphology for up to 2 weeks. These assays are flexible, simple to run and provide automated and direct measures of tumor size in real-time.
Related technologies: BioImaging
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