Discover

Back to filter

Related topics

Early immune response in mice immunized with inactivated vaccine against SARS-CoV-2

Cytek Biosciences

Nov 23, 2020

The development of a vaccine against COVID-19 is a hot topic for many research laboratories all over the world. Our aim...

CS-FNX Hitachi Koki, discover the state-of-art ultracentrifuges series

Hitachi Koki

Nov 19, 2020

Hitachi Koki, as a world leading producer of centrifuges offers general purpose floor standing and table top...

Curiox webinar - how to retain more cells for Single Cell Sequencing and proteogenomics ?

Curiox

Nov 19, 2020

Do you think that centrifuging cells at 500 x g is not going to cause issues? Moreover, sample prep involving...

Discover the new NovoCyte Penteon - when exceptional performance meets simplicity

Agilent technologies

Nov 18, 2020

The NovoCyte Penteon flow cytometer builds on its successful predecessor, the NovoCyte. You now have the flexibility to...

Introducing the GenapSys Sequencer

GenapSys

Apr 15, 2020

GenapSys has developed a novel electrical-based platform capable of accurately detecting single base incorporations....

Cellular stress induces erythrocyte assembly and promotes microangiopathy

Fluxion Biosciences

Dec 19, 2018

Microangiopathy with subsequent organ damage represents a major complication in several diseases. The mechanisms...

The need for ultra high field magnetic resonance imaging

Bruker Biospin

Dec 17, 2018

MRI  is a noninvasive imaging technology that is known for its superior soft tissue contrast and ability to provide...

NovoCyte Quanteon webinar: In-depth cell Proliferation through new advances in flow cytometry

Agilent technologies

Dec 11, 2018

Cell proliferation is one of the most fundamental biological processes in biology. Many of the traditional assays to...

Webinar: early detection of metabolic remodeling in pressure overload left ventricle Hypertrophy

Bruker Biospin

Nov 28, 2018

Do you already heard the hypothesis that metabolic changes in the heart may precede and even trigger structural and...

IncuCyte neuronal activity assay

Sartorius

Nov 13, 2018

The generation and maturation of neuronal networks are necessary characteristics of neurogenesis, providing...

Show all topics (10)

Label-free, real-time live-cell assays for spheroids: IncuCyte bright-field analysis

Feb 12, 2018

Current methods for assessing the growth and shrinkage  of  tumor  spheroids  are  limited  by  one  or  more  of  the  following:  (1)  assay  workflows  that  are  time-consuming,  expensive  or  laborious,  (2)  a  requirement  to  label  the  cells  (e.g.  a  fluorescent  probe)  which  may  perturb  the  biology  and  may  not  be  amenable  to  primary  tissue,  (3)  single  time-point  readouts  that  do  not  report  the  full  timecourse,  (4)  indirect  readouts (e.g. ATP) that may overlook  valuable morphological insight and/or mis-report cell growth.In  this  application  note  we  describe  methods  and  validation  data  for  miniaturized  (96/384-well)  live-cell  tumor  spheroid  assays   that   are   based   on   non-invasive   bright-field   image   analysis  performed  with  the  IncuCyte  S3  Spheroid  software  module.  Tumor  spheroids  are  formed  in  ultra-low  attachment  (ULA) plates and monitored for size and morphology for up to 2  weeks.  These  assays  are  flexible,  simple  to  run  and  provide  automated and direct measures of tumor size in real-time.

Read more

 

Scientific paper
Application
Product news

Related technologies: BioImaging

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

Related products

High throughput, real-time and automated measurements of live-cell activity, inside your incubator

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey