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Recent publication of in-vivo two-photon intravital imaging study targeting mouse kidney

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In vivo longitudinal 920 nm two-photon intravital kidney imaging of a dynamic 2,8-DHA crystal
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Webinar: Multimodal tissue imaging and machine learning to advance precision medicine

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Real-Time and Quantitative Analysis of Macrophage Phagocytosis with RTCA eSight

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Revolutionize your Flow Cytometry and Sorting workflow with Cytek Biosciences

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A deep learning and Monte Carlo based framework for bioluminescence imaging center Maastro

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Morphological and pharmacological in-vitro kinetic angiogenesis assays

Nov 16, 2016

The ADSC/ECFC model yields rapidly forming (<48h) endothelial cell‘cord’structures. In the NHDF/HUVEC slowly forming‘tube-like’structures appear which continue to develop and branch even after 10days in culture. High basal formation was observed in the ADSC/ECFC model, but not in the NHDF/HUVEC model. From immuno-cytochemistry, the ADSCs surrounding the cord network label for PDGFR-β and a-SMA, suggesting apericyte phenotype.The pharmacological effects of growth factors and different pathway inhibitors were largely comparable. Interestingly, established cords and tubes display marked resistance to Avastin (Bevacizumab) compared to developing networks. G-secretase inhibition partially reversed established tubes in ADSC/ECFCs and augmented late state branching in the NHDF/HUVEC model. We conclude that these 2 models exhibit strikingly different morphological, temporal and pharmacological profiles. The resistance of established vascular structures to disruption by Avastin (Bevazicumab) may represent a useful translational paradigm for addressing tumour resistance of anti-VEGF therapies.

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