Discover

Back to filter

Related topics

Automatic, Real Time Acquisition of Bioluminescent Kinetic Curves

Spectral Instruments Imaging

Jun 27, 2024

Watch this pre-recorded webinar with Dr. Andrew Van Praagh to learn how our new Aura software feature —Kinetics—...

Theranostics: From Mice to Men and Back

MOLECUBES

Jun 25, 2024

Recorded webinar
Presenters: Prof. Dr. Ken Herrmann and Prof. Dr. Katharina Lückerath – Moderator: Hannah Notebaert

Orion 2024 AACR poster: 17-plex single-step stain and imaging of cell Lung Carcinoma

RareCyte

Jun 21, 2024

RareCyte Orion is a benchtop, high resolution, whole slide multimodal imaging instrument. A combination of quantitative...

New release now available: Cytek Amnis AI v3.0 Software

Cytek Biosciences

Jun 17, 2024

The new Cytek Amnis AI v3.0 image analysis software features an integrated transfer learning algorithm, an option to...

Automated Purification of Viral DNA and RNA from Biological Samples usingZymo Research Quick-DNA/RNA

Analytik Jena

Jun 14, 2024

The Quick DNA/RNA Viral MagBead Kit from Zymo has been automated with the Cybio FeliX pipetting robot from Analytik...

DNA Amplicon Library Preparation for Illumina® Sequencing

Analytik Jena

Jun 12, 2024

The precision of temperature control, efficient heating and cooling rates, and excellent temperature homogeneity across...

Cytek webinar: Imaging Flow Cytometry for Chromosomal Assessment in Hematological Malignancies

Cytek Biosciences

Jun 7, 2024

In this webinar, we will describe a new innovative approach we developed that resolves these limitations. The method...

X-RAD 320 for irradiation therapy during quantifying study for in vivo collagen reorganization

Precision X-Ray

Jun 5, 2024

Quantifying in vivo collagen reorganization during immunotherapy in murine melanoma with second harmonic generation...

Exosome-Mediated Delivery of Small Molecules, RNA & DNA for Development of Novel Cancer Therapeutics

Spectral Instruments Imaging

Jun 3, 2024

Disha Moholkar of University of Louisville's Gupta Lab
Tuesday, June 11, 2024, 6:30 PM
...

Emulate in vivo conditions – introduce shear flow to your experiments with BioFlux system

Cell Microsystems

May 27, 2024

Most research is still conducted in vitro without the presence of flow. We use the BioFlux System to give you the...

Show all topics (10)

Neuronal co-culture - fluorescent live cell imaging

Aug 4, 2016

Neurite dynamics can be altered in disease states or following exposure to neurotoxic agents providing a rationale for understanding and quantifying this process (Conforti et al., 2007). An ideal method to track neurite dynamics would allow continuous automated measurement of structural parameters, including length and number of branch points, in a non-perturbing manner. We have previously developed a quantitative mono-culture approach for investigating changes in neurite length and ranching using phase images taken with an IncuCyte ZOOM live-content imaging platform in conjunction with NeuroTrack analysis software. However, in co-cultures containing neurons and astrocytes, neuronal processes cannot be unambiguously identified and measured using phase images alone. As such, we have developed a fluorescent reagent (NeuroLight Red) that specifically labels neurons in co-culture with astrocytes. Utilising a VSV-G pseudotyped lentivirus that encodes a red fluorescent protein (mKate2) driven off a synapsin promoter, neuronal expressionis strengthened and non-neuronal crossover is minimized. Here we describe assay methods using this fluorescent label, alongside NeuroTrack algorithms, to quantitatively assess changes in neurite morphology over time in either rat primary or human iPSC-derived neuron/astrocyte co-cultures.

Read more

 

Scientific paper
Application
Product news

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey