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Discover the Celloger live-cell analyzers : miniaturized and powerful tools for every Biologist

Curiosis

Apr 5, 2024

Celloger live-cell analyzers, image and analyze your cells in real-time, inside your incubator. With its exceptional...

The chicken chorioallantoic membrane as a low-cost, high-throughput model for cancer imaging

Precision X-Ray

Apr 4, 2024

Here, we assessed the chicken chorioallantoic membrane (CAM) as an alternative to mice for preclinical cancer imaging...

A microthrombus-driven fixed-point cleaved nanosystem for preventing post-thrombolysis recurrence

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Apr 3, 2024

A thrombin-responsive and fixed-point cleaved Fu@pep-CLipo was developed for highly efficient and precise thrombolysis...

Multiplexed tissue imaging using the Orion platform to reveal the Spatial Biology of Cancer

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Mar 27, 2024

In this webinar Prof. Sandro Santagata, will reveal how Orion high-plex imaging and the use of this data, is valuable...

Cytek SpectroFlo Software Version 3.3 has been released!

Cytek Biosciences

Mar 25, 2024

SpectroFlo Software Version 3.3 has been released, enabling new assays with SpectroFlo Software version 3.3, including...

Innovation is in its DNA

Mission Bio

Mar 22, 2024

Mission Bio’s Tapestri Tool May Hold the Key to Informing Treatment Options for Multiple Myeloma.

A 19-color single-tube Full Spectrum Flow Cytometry for the detection of Acute Myeloid Leukemia

Cytek Biosciences

Mar 13, 2024

This recent publication in Cytometry Part A describes the development and comprehensive workflow of a single-tube,...

18F-labeled somatostatin analogs for somatostatin receptors (SSTRs) targeted PET imaging of NETs

MOLECUBES

Mar 11, 2024

A novel 18F-radiolabeled somatostatin analogue, [Al18F]NODA-MPAA-HTA, was synthesized and evaluated for positron...

Discover Yokogawa CellVoyager CQ1: Benchtop High-Content Analysis System

Yokogawa

Mar 8, 2024

Unlike flow cytometers, the CellVoyager CQ1 confocal quantitative image cytometer does not require pretreatment such as...

Real-time and quantitative analysis of Macrophage Phagocytosis with RTCA eSight

Agilent technologies

Feb 23, 2024

The eSight is currently the only instrument that interrogates cell health and behavior using cellular
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Neuronal co-culture - fluorescent live cell imaging

Aug 4, 2016

Neurite dynamics can be altered in disease states or following exposure to neurotoxic agents providing a rationale for understanding and quantifying this process (Conforti et al., 2007). An ideal method to track neurite dynamics would allow continuous automated measurement of structural parameters, including length and number of branch points, in a non-perturbing manner. We have previously developed a quantitative mono-culture approach for investigating changes in neurite length and ranching using phase images taken with an IncuCyte ZOOM live-content imaging platform in conjunction with NeuroTrack analysis software. However, in co-cultures containing neurons and astrocytes, neuronal processes cannot be unambiguously identified and measured using phase images alone. As such, we have developed a fluorescent reagent (NeuroLight Red) that specifically labels neurons in co-culture with astrocytes. Utilising a VSV-G pseudotyped lentivirus that encodes a red fluorescent protein (mKate2) driven off a synapsin promoter, neuronal expressionis strengthened and non-neuronal crossover is minimized. Here we describe assay methods using this fluorescent label, alongside NeuroTrack algorithms, to quantitatively assess changes in neurite morphology over time in either rat primary or human iPSC-derived neuron/astrocyte co-cultures.

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