Optical tweezers, Confocal microscopy, STED nanoscopy, Acoustic force spectroscopy for single-molecule biophysics.
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Novel insights on the mechanical properties of IFs using LUMICKS’ technology
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IFs are one of the filament systems which constitute the cytoskeleton of the eukaryotic cell. Up until now very little was known regarding the physical properties and their roles in cellular mechanics, despite being associated with metastasis and being overexpressed in cancerous cells. In this article the optical tweezers – fluorescence microscopy in combination with computational methods were used to trap individual vimentin filaments and investigate their mechanical properties. The authors obtained insights into the energy dissipation, strain history dependence, and creep behavior of stretch filaments after which they linked these experimental observations to the known molecular architecture of IFs.
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