Discover

Back to filter

Related topics

Pretargeted delivery of PI3K/mTOR small-molecule inhibitor–loaded nanoparticles

Spectral Instruments Imaging

Jun 23, 2021

Overactivation of the PI3K/mTOR signaling has been identified in non-Hodgkin’s lymphoma....

Top 10 Pharma companies have chosen CYTEK for their flow cytometry facility

Cytek Biosciences

Jun 21, 2021

6 of Top 10 Pharma Companies (Based on total group revenues) have chosen CYTEK - full...

Elevated NSD3 histone methylation activity drives squamous cell lung cancer

Spectral Instruments Imaging

Jun 8, 2021

This work identifies NSD3 as a principal 8p11-12 amplicon-associated oncogenic driver in...

A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic

Tonbo Biosciences

May 21, 2021

Tonbo Biosciences flow cytometry reagents & antibodies are manufactured with the highest...

IncuCyte webinar recording: How to measure Immune Cell Killing of Tumor Cells effectively

Sartorius

May 19, 2021

Immuno-oncology (IO) has transformed cancer treatment. The number of treatments in the IO...

White Paper: Immunophenotyping Rare Immune Cells with Laminar Wash AUTO System

Curiox

May 18, 2021

The Laminar Wash (LW) AUTO system consists of a wall-less plate and a laminar flow cell...

Show all topics (10)

Platelet GPIIb supports initial pulmonary retention but inhibits subsequent proliferation of melanom

Jul 28, 2017

In the present study, the research team dissected the role of platelet αIIb integrin (GPIIb) for early and late steps in pulmonary melanoma metastasis. They first addressed in vitro potential mechanisms for initial recruitment of circulating melanoma cells to vascular endothelium using a flow chamber model and then assessed the role of GPIIb for metastasis formation in vivo in mice lacking integrin αIIb (GPIIb-/-). GPIIb associates with GPIIIa (integrin β3) to form the platelet-specific integrin heterodimer GPIIb-IIIa (integrin αIIbβ3), representing the most abundant platelet surface receptor and predominantly functioning as platelet fibrinogen receptor. By binding to fibrinogen, but also to von Willebrand factor, GPIIb-IIIa mediates cross-linking of adjacent platelets, resulting in platelet aggregation and platelet secretion of chemokines as well as growth factors. Moreover, binding of GPIIb-IIIa to fibronectin, vitronectin or PECAM-1 leads to platelet adhesion to the vessel wall. In order to follow the initial steps of tumor metastasis in wildtype (WT) and GPIIb-deficient mice, they applied a novel microscopic approach using a fluorescence optical imaging system based on laser scanning confocal technology.

They showed that the acute retention of malignant melanoma cells is dramatically reduced in mice deficient in platelet GPIIb and also they found that GPIIb has a minor effect of adhesion of single melanoma cells, but rather mediates the formation of platelet-rich melanoma cell aggregates, which are retained in the pulmonary vasculature.

Read more

Scientific paper
Application

Brand profile

Mauna Kea Technologies

Mauna Kea Technologies manufactures Cellvizio Lab, confocal probe based imaging system, providing cellular-level images with minimal invasiveness for longitudinal studies.

Related products

Cellvizio, a fiber based microscope for observing in real time at cellular scale, features simultaneous, co-registered dual band excitation and detection

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey