Discover

Back to filter

Related topics

Curiox webinar - how to retain more cells for Single Cell Sequencing and proteogenomics ?

Curiox

Feb 15, 2021

Curiox Laminar Wash technology has been added as part of the official BioLegend TotalSeq...

Cytek Aurora in Action : how to easily resolve highly overlapping dyes ?

Cytek Biosciences

Feb 2, 2021

Learn from Jeff Nelson, a manager of a cytometry shared resource laboratory, about the...

Webinar: A simple Migration/Invasion workflow using IncuCyte Live-cell analysis system

Sartorius

Jan 27, 2021

Cell migration and invasion, or cell motility, play a role in many normal and...

Webinar : meet the Cytek's new cFluor reagents

Cytek Biosciences

Jan 25, 2021

Cytek Biosciences has already redefined what is possible in flow cytometry. The new Cytek...

Introducing Cytek cFluor, 14 Colors Immunoprofiling Kit

Cytek Biosciences

Jan 19, 2021

Cytek cFluor Immunoprofiling Kit allows for the identification of helper T cells,...

Cytek full Spectrum flow Cytometry webinar: Part II – panel design and troubleshooting

Cytek Biosciences

Jan 11, 2021

In this session, we will continue to look at full spectrum flow cytometry and look at considerations for fluorescence...

IncuCyte for in-vitro analysis of SARS-CoV-2 vaccine candidate

Sartorius

Jan 4, 2021

Watch this video to learn how researcher at Humane Genomics are utilizing the Incucyte...

Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice

Precision X-Ray

Dec 29, 2020

Radio-active sources have been used routinely for the preconditioning in humanized mouse...

Advances in Leukemia research using shear flow and Bioflux system

Fluxion Biosciences

Dec 10, 2020

Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes...

Do you have our CellRad benchtop irradiator? It’s the only one on the market!

Precision X-Ray

Dec 9, 2020

The CellRad is a smaller, simpler, safer, and more cost effective alternative to...

Show all topics (10)

Platelet GPIIb supports initial pulmonary retention but inhibits subsequent proliferation of melanom

Jul 28, 2017

In the present study, the research team dissected the role of platelet αIIb integrin (GPIIb) for early and late steps in pulmonary melanoma metastasis. They first addressed in vitro potential mechanisms for initial recruitment of circulating melanoma cells to vascular endothelium using a flow chamber model and then assessed the role of GPIIb for metastasis formation in vivo in mice lacking integrin αIIb (GPIIb-/-). GPIIb associates with GPIIIa (integrin β3) to form the platelet-specific integrin heterodimer GPIIb-IIIa (integrin αIIbβ3), representing the most abundant platelet surface receptor and predominantly functioning as platelet fibrinogen receptor. By binding to fibrinogen, but also to von Willebrand factor, GPIIb-IIIa mediates cross-linking of adjacent platelets, resulting in platelet aggregation and platelet secretion of chemokines as well as growth factors. Moreover, binding of GPIIb-IIIa to fibronectin, vitronectin or PECAM-1 leads to platelet adhesion to the vessel wall. In order to follow the initial steps of tumor metastasis in wildtype (WT) and GPIIb-deficient mice, they applied a novel microscopic approach using a fluorescence optical imaging system based on laser scanning confocal technology.

They showed that the acute retention of malignant melanoma cells is dramatically reduced in mice deficient in platelet GPIIb and also they found that GPIIb has a minor effect of adhesion of single melanoma cells, but rather mediates the formation of platelet-rich melanoma cell aggregates, which are retained in the pulmonary vasculature.

Read more

Scientific paper
Application

Brand profile

Mauna Kea Technologies

Mauna Kea Technologies manufactures Cellvizio Lab, confocal probe based imaging system, providing cellular-level images with minimal invasiveness for longitudinal studies.

Related products

Cellvizio, a fiber based microscope for observing in real time at cellular scale, features simultaneous, co-registered dual band excitation and detection

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey