Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
Automated microscopy and Spatial Proteomics
Automated microscopy and image analysis
Discover
Related topics
Factors Affecting Petri Dish Condensation in Tissue Culture (CU) Chambers
Nov 29, 2023
In this report, we will investigate the effects of infrared lighting, light intensity, stacking, as well as constant...
Visualization of spatial distribution of hemoglobin with various oxygen saturations in small animals
Nov 28, 2023
With the aid of our uniquely developed device and analysis software, our primary objective is to map the spatial...
A fluorescent reporter system for anaerobic thermophiles
Nov 27, 2023
Anaerobic microorganisms are key effectors for the sustainable production of biofuels and biochemicals, as they...
Vizgen webinar: spatial relationships in Developmental Biology
Nov 21, 2023
MERFISH integrates spatial transcriptomics technology with high resolution spatial imaging, In this webinar we will...
Nov 14, 2023
The xCELLigence RTCA eSight enables simultaneous real time biosensor impedance-based and...
NanoCellect webinar: Plant Potential - Gentle Cell Sorting for Enhanced Plant Biology Workflows
Nov 3, 2023
Gentle cell sorting is a useful tool to increase the efficiency of plant biology workflows that include gene...
SIMOA-based analysis of plasma NFL levels in MCI and AD patients
Nov 3, 2023
The single-molecule array assay (SIMOA)-based detection of neurofilament light (NFL) chain could be useful in...
Single Cell Deposition: the cornerstone of Flow Cytometry for cellular analysis and manipulation
Nov 2, 2023
One notable technology for harnessing the power of single cell deposition is the NanoCellect WOLF G2 Cell Sorter and N1...
Single cell genotypic and phenotypic analysis of residual disease in acute myeloid leukemia
Nov 1, 2023
Measurable residual disease (MRD), defined as the population of cancer cells which persists following therapy, serves...
Emulate in vivo conditions – introduce shear flow to your experiments with BioFlux system
Oct 31, 2023
Most research is still conducted in vitro without the presence of flow. We use the BioFlux System to give you the...
May 4, 2023
Considering the critical roles that phagocytosis plays in microbial defense, wound healing, development,
and tissue homeostasis, it is not surprising that numerous assays have been developed to study phagocytosis in vitro. The primary legacy assays involve microscopy, flow cytometry, or spectrophotometry/spectrofluorimetry. In these assays phagocytes are typically incubated for a fixed period with particles (dextran, zymosan, E. coli) that are labeled to be constitutively fluorescent, followed by endpoint analysis. Quantification of phagocytosis by microscopy often requires cell fixation followed by manually counting the number of particles inside each cell, which is tedious and not amenable to
high throughput studies. Quantification of phagocytosis by spectrofluorimetry requires that uningested particles either be quenched or physically removed before analysis. Variations on these
assays, where particles are coupled to enzymes that are detectable using colorimetric substrates once cells have been fixed and permeabilized, still require a significant amount of hands-on time. The same is also true of flow cytometry‑based assays where phagocytes must be collected from the well bottom before they can be analyzed. In contrast to all these legacy methods, the eSight assay described here only requires mixing phagocytes with substrates; zero handing steps are subsequently required. Another distinguishing feature of this eSight phagocytosis assay is its continuous nature. Tracking phagocytosis in real time makes it possible to focus analyses on the most appropriate time regions.
Related technologies: Live cell imaging
Get more info
Brand profile
Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
More info at:
www.aceabio.com