Discover

Back to filter

Related topics

qPCR? Learn more about qTOWER³ Product Family – Product Animation

Analytik Jena

Apr 12, 2021

The qTOWER³ product family guarantees well-founded real-time results as it benefits from peerless temperature control...

SARS-CoV-2 N Protein Antigen Test as new Quanterix tool in ultrasensitive biomarkers detection

Quanterix

Apr 12, 2021

Digital health solution, Simoa, is changing the way in which the biology of health and disease is studied by giving...

Observe the movement of live cells that make up living tissue in vivo!

IVIM Technology

Apr 12, 2021

IntraVital Microscopy is a technique that enables you to directly observe the movement of live cells that make up...

Single-cell DNA Analysis of a Novel Acute Erythroid Leukemia CRISPR Induced Mouse Model

Mission Bio

Apr 12, 2021

Single-cell DNA Analysis of a Novel Acute Erythroid Leukemia (AEL) CRISPR Induced Mouse Model.
Assessment of
...

Developing tools for multiplex to single EV analysis pipelines

Cytek Biosciences

Apr 12, 2021

Extracellular vesicles (EVs) are lipid spheres released from cells. EVs express proteins...

SARS-CoV-2 detection workflow in wastewater- Analytik Jena and project in Japan

Analytik Jena

Apr 12, 2021

In 2020 Analytik Jenas real-time PCR thermal cyclers of the qTOWER³ series even supported one of the first SARS-CoV-2...

Observe the movement of live cells that make up living tissue in vivo

IVIM Technology

Apr 8, 2021

IntraVital Microscopy is a technique that enables you to directly observe the movement of...

Cytek webinar : Improved flow cytometry assay resolution with autofluorescence extraction

Cytek Biosciences

Mar 30, 2021

The power of autofluorescence extraction in the detection of dim fluorescence on highly...

Innovation never takes a break with IncuCyte

Sartorius

Mar 11, 2021

Analyze your cells for days, weeks or even months as they sit stationary in the stable...

Show all topics (10)

Real-time quantitative analysis of apoptosis

Jun 21, 2016

Apoptosis is an essential process for normal tissue development and homeostasis by which cells undergo timely programmed cell death. Aberrations in apoptotic signaling are implicated in a range of human pathologies including cancer, autoimmune disease and neurodegeneration. Induction of apoptosis leads, in most cases, to the activation of caspases (cysteinyl aspartate proteinases) and plasma membrane alterations. The activation of caspase-3 or caspase-7 results in the irreversible commitment of the cell to apoptotic death, and is considered a reliable marker for apoptosis. The regulated loss of plasma membrane phosphatidylserine (PS) symmetry is also a classical marker of apoptosis. Dying cells trigger the translocation of the normally inward-facing PS to the cellular surface, allowing for early phagocytic recognition of the dying cell by surrounding phagocytes.

The IncuCyte Annexin V Reagents are highly-selective phosphatidylserine (PS) cyanine fluorescent dyes. Specially formulated, these reagents are ideally suited for real time measurements of apoptosis in living cultures. They are validated for use with the IncuCyte ZOOM live cell analysis system and employ simple mix-and-read protocols. Addition of the IncuCyte Annexin V Reagents to normal healthy cells is non-perturbing to cell growth and morphology and yields little or no intrinsic fluorescent signal. Once cells become apoptotic plasma membrane PS asymmetry is lost. PS exposure on the extracellular surface enables binding of the IncuCyte Annexin V Reagent resulting in a bright and photostable fluorescent signal. With the IncuCyte ZOOM integrated analysis software fluorescent apoptotic cells can be quantified and background fluorescence minimized.

IncuCyte Annexin V Reagents enable real-time evaluation of PS exposure in response to pharmacological agents and/or genetic and environmental factors. Furthermore, the IncuCyte Annexin V Reagents can be combined with the IncuCyte ZOOM confluence metric, IncuCyte Caspase 3/7 Reagent, IncuCyte NucLight nuclear labeling reagents or IncuCyte Cytotox Reagents for multiplexed measurements of apoptosis, cell proliferation or cytotoxicity in every assay well.

Application
Product news

Related technologies: BioImaging

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey