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Cytek full Spectrum flow Cytometry webinar: Part II – panel design and troubleshooting

Cytek Biosciences

Jan 11, 2021

In this session, we will continue to look at full spectrum flow cytometry and look at considerations for fluorescence...

IncuCyte for in-vitro analysis of SARS-CoV-2 vaccine candidate

Sartorius

Jan 4, 2021

Watch this video to learn how researcher at Humane Genomics are utilizing the Incucyte platform for the in-vitro...

Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice

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Radio-active sources have been used routinely for the preconditioning in humanized mouse models, but safety issues have...

Coming in spring 2021, meet the Cytek Aurora CS

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Ever wanted to take your full spectrum panel from your Cytek Aurora or Northern Lights cytometer and sort? Soon, you...

Advances in Leukemia research using shear flow and Bioflux system

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Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes create disruptions in the immune...

Do you have our CellRad benchtop irradiator? It’s the only one on the market!

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The CellRad is a smaller, simpler, safer, and more cost effective alternative to radioisotope or high-powered X-ray...

Dosimetric characterization of an X-ray irradiator for use with cells

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This study aims to characterize an X-ray irradiator for use with cells using its internal parallel-plate ionization...

Curiox - how to retain more cells for Single Cell Sequencing and proteogenomics ?

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Do you think that centrifuging cells at 500 x g is not going to cause issues? Moreover, sample prep involving...

CS-FNX Hitachi Koki, discover the state-of-art ultracentrifuges series

Hitachi Koki

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Hitachi Koki, as a world leading producer of centrifuges offers general purpose floor standing and table top...

Discover the new NovoCyte Penteon - when exceptional performance meets simplicity

Agilent technologies

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The NovoCyte Penteon flow cytometer builds on its successful predecessor, the NovoCyte. You now have the flexibility to...

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Real-time quantitative analysis of apoptosis

Jun 21, 2016

Apoptosis is an essential process for normal tissue development and homeostasis by which cells undergo timely programmed cell death. Aberrations in apoptotic signaling are implicated in a range of human pathologies including cancer, autoimmune disease and neurodegeneration. Induction of apoptosis leads, in most cases, to the activation of caspases (cysteinyl aspartate proteinases) and plasma membrane alterations. The activation of caspase-3 or caspase-7 results in the irreversible commitment of the cell to apoptotic death, and is considered a reliable marker for apoptosis. The regulated loss of plasma membrane phosphatidylserine (PS) symmetry is also a classical marker of apoptosis. Dying cells trigger the translocation of the normally inward-facing PS to the cellular surface, allowing for early phagocytic recognition of the dying cell by surrounding phagocytes.

The IncuCyte Annexin V Reagents are highly-selective phosphatidylserine (PS) cyanine fluorescent dyes. Specially formulated, these reagents are ideally suited for real time measurements of apoptosis in living cultures. They are validated for use with the IncuCyte ZOOM live cell analysis system and employ simple mix-and-read protocols. Addition of the IncuCyte Annexin V Reagents to normal healthy cells is non-perturbing to cell growth and morphology and yields little or no intrinsic fluorescent signal. Once cells become apoptotic plasma membrane PS asymmetry is lost. PS exposure on the extracellular surface enables binding of the IncuCyte Annexin V Reagent resulting in a bright and photostable fluorescent signal. With the IncuCyte ZOOM integrated analysis software fluorescent apoptotic cells can be quantified and background fluorescence minimized.

IncuCyte Annexin V Reagents enable real-time evaluation of PS exposure in response to pharmacological agents and/or genetic and environmental factors. Furthermore, the IncuCyte Annexin V Reagents can be combined with the IncuCyte ZOOM confluence metric, IncuCyte Caspase 3/7 Reagent, IncuCyte NucLight nuclear labeling reagents or IncuCyte Cytotox Reagents for multiplexed measurements of apoptosis, cell proliferation or cytotoxicity in every assay well.

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Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. They invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

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