Discover

Back to filter

Related topics

Understand the Cancer Ecosystem based on single cells analysis

Mission Bio

Sep 22, 2022

Mission Bio is a life sciences company that accelerates discoveries and cures for a wide range of diseases by equipping...

Automating DNA Extraction for High-Throughput Bacterial Plasmid Purification with CyBi®-SELMA

Analytik Jena

Sep 19, 2022

The Need to Increase Plasmid Isolation Throughput, Challenges with Scaling up Plasmid DNA Extraction, Automating the...

Introducing RareCyte Orion, integrating Spatial Biology with Multiplex Imaging

RareCyte

Sep 13, 2022

Orion, the highly multiplexed fluorescent imaging system, empower investigators to unlock spatial context for key...

Cytek Aurora at CIVIS Summer School

Cytek Biosciences

Aug 23, 2022

OMIXYS´s workshop during CIVIS project for spectral flowcytometry, using Aurora from Cytek

xCELLigence eSight - Imaging & Impedance in real-time inside your incubator

Agilent technologies

Aug 22, 2022

The xCELLigence RTCA eSight enables comprehensive insight into cell health, cell behavior, cell function and cell...

Standardize tumor sample preparation and reduce background with Curiox laminar wash system

Curiox

Aug 10, 2022

Laminar Wash technology offers a gentle alternative that can improve yield and data quality, particularly of rare cell...

Developing a 43 color panel on Cytek Aurora using Laminar Wash for sample prep

Curiox

Aug 2, 2022

Successfully executing a 43 color panel is a technically demanding task. One that is made more difficult when there are...

Transcriptome-Informed Approach to Drug Discovery

HTG Molecular Diagnostics

Jul 12, 2022

The HTG EdgeSeq system is a powerful molecular profiling tool for biopharmaceutical companies, academic research...

Semi-automated genomic DNA extractionfrom Plant with CyBi®-SELMA

Analytik Jena

Jul 12, 2022

Plant genomic DNA extraction with CyBio SELMA, Semi-Automated Pipette with minimal benchtop footprint

New Center of Excellence, located at the Josep Carreras Leukaemia Research Institute

Mission Bio

Jul 12, 2022

Mission Bio is a life sciences company that accelerates discoveries and cures for a wide range of diseases by equipping...

Show all topics (10)

The effects of hypoxia and hydrostatic pressure in translational research

Apr 16, 2018

The characterization of the tumor microenvironment includes mechanotransduction, hypoxia, acidosis, and tissue remodeling. These factors can influence the gene and protein expression of variouse cell types that make up the tumor as well as influence the selection of cells that can thrive in a given microenvironment; however, typical cancer cell culture rarely uses hypoxia and pressure onr utilizes substrates similar to the native extracellular matrix. Cancer cell lines and PBMCs differentially express immunotherapeutic targets, at low oxygen and high pressure culturing conditions, resulting in reduced expression of key drug targets.

Analysis of mRNA-seq data revealed alterations in gene expression profiles of immunotherapeutic and drug-target pathways involving CTLA-4 and AR signaling. In contrast, it was obsorved increased CD47 and CD44 expression at low oxzgen and high presure culturing conditions in cancer cell lines and immune cells. Therefore these results support the presence of physiologically-relevant drug targets in tumor cells characterized by low oxygen and high interstitial fluid preassure.

Application
Product news

Brand profile

Xcell Biosciences

The company provides a revolutionary cell culturing platform that replicates the physiological environment of native tissues to deliver reproducible and biologically relevant molecular analysis.

Related products

The Avatar system can mimic the oxygen and pressure levels of human tissues

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey