Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
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Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice
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Radio-active sources have been used routinely for the preconditioning in humanized mouse...
Advances in Leukemia research using shear flow and Bioflux system
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Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes...
Do you have our CellRad benchtop irradiator? It’s the only one on the market!
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Dosimetric characterization of an X-ray irradiator for use with cells
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Early immune response in mice immunized with inactivated vaccine against SARS-CoV-2
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The development of a vaccine against COVID-19 is a hot topic for many research...
Cellular stress induces erythrocyte assembly and promotes microangiopathy
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Microangiopathy with subsequent organ damage represents a major complication in several diseases. The mechanisms...
The need for ultra high field magnetic resonance imaging
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The most advanced software for preclinical imaging research
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Modern imaging laboratories hosting PET/MR instruments profit from the fully integrated common imaging platform...
Red blood cell-hitchhiking boosts delivery of nanocarriers to chosen organs
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Drug delivery by nanocarriers (NCs) has long been stymied by dominant liver uptake and limited target organ deposition,...
Adenovirus type B detection using the xxpress qPCR thermal cycler
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The ultrafast xxpress qPCR thermal cycler through performing standard dilution series and multiplex qPCR experiments is...
A novel real-time CTL assay to measure designer T-cell function against HIV Env(+) cells
Feb 19, 2018
Stimulated rhesus PBMCs with antibodies to CD3/CD28 and cotransduced T cells with CD4-CAR and maC46 vectors. CD4-CAR-transduced T cells were added to Env(+) 293T cells at E:T of 1:1. Killing of target cells was measured as reduced impedance. Has been observed gene expression in 60-70% of rhesus CD3(+) CD8(+) T cells with the individual vectors and in 35% of the cells with both vectors. CD4-CAR-transduced populations specifically killed Env(+) cells. In these studies, scientist showed that designer T cells were redirected to kill Env(+) cells. Control of viremia without HAART would revolutionize treatment for HIV patients.
Related technologies: Conventional flow cytometry
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