Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
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Automating DNA Extraction for High-Throughput Bacterial Plasmid Purification with CyBi®-SELMA
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The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting...
Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters
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Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters, whereas hydroxychloroquine...
Quantitative 3D Optical Imaging for Lago
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Extensive assessment of Cytokine production on the NovoCyte Penteon flow cytometer
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How to Improve Your Viral Nucleic Acid Extraction Processes
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Comparison of gamma and x-ray irradiation results
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Biological effects of Cs-137 and X-ray irradiators are comparable with regards to...
Hypoxia further exacerbates myopathy in broilers via alteration of satellite cell fate
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A novel real-time CTL assay to measure designer T-cell function against HIV Env(+) cells
Feb 19, 2018
Stimulated rhesus PBMCs with antibodies to CD3/CD28 and cotransduced T cells with CD4-CAR and maC46 vectors. CD4-CAR-transduced T cells were added to Env(+) 293T cells at E:T of 1:1. Killing of target cells was measured as reduced impedance. Has been observed gene expression in 60-70% of rhesus CD3(+) CD8(+) T cells with the individual vectors and in 35% of the cells with both vectors. CD4-CAR-transduced populations specifically killed Env(+) cells. In these studies, scientist showed that designer T cells were redirected to kill Env(+) cells. Control of viremia without HAART would revolutionize treatment for HIV patients.
Related technologies: Conventional flow cytometry
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