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Extensive assessment of Cytokine production on the NovoCyte Advanteon flow cytometer

Agilent technologies

Jan 13, 2023

Cytokines are small molecules essential for immune cell response to activation by pathogens, autoimmunity, or...

Microfluidic cell sorter sample preparation for genomic assays

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Transcriptome-Informed Approach to Drug Discovery

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Semi-automated genomic DNA extractionfrom Plant with CyBi®-SELMA

Analytik Jena

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Plant genomic DNA extraction with CyBio SELMA, Semi-Automated Pipette with minimal benchtop footprint

Outstanding post-sort sample recovery on the WOLF Cell Sorter

NanoCellect

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The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting...

Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters

MOLECUBES

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Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters, whereas hydroxychloroquine...

Quantitative 3D Optical Imaging for Lago

Spectral Instruments Imaging

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Spectral Instrument Imaging just announced a new, strategic partnership with InVivo Analytics, which enables...

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A novel real-time CTL assay to measure designer T-cell function against HIV Env(+) cells

Feb 19, 2018

Stimulated rhesus PBMCs with antibodies to CD3/CD28 and cotransduced T cells with CD4-CAR and maC46 vectors. CD4-CAR-transduced T cells were added to Env(+) 293T cells at E:T of 1:1. Killing of target cells was measured as reduced impedance. Has been observed gene expression in 60-70% of rhesus CD3(+) CD8(+) T cells with the individual vectors and in 35% of the cells with both vectors. CD4-CAR-transduced populations specifically killed Env(+) cells. In these studies, scientist showed that designer T cells were redirected to kill Env(+) cells. Control of viremia without HAART would revolutionize treatment for HIV patients.

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Scientific paper

Related technologies: Conventional flow cytometry

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Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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