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Introducing the GenapSys Sequencer

GenapSys

Apr 15, 2020

GenapSys has developed a novel electrical-based platform capable of accurately detecting single base incorporations....

IncuCyte neuronal activity assay

Sartorius

Nov 13, 2018

The generation and maturation of neuronal networks are necessary characteristics of neurogenesis, providing...

COY - personalized hypoxic chambers

COY laboratory products

Oct 18, 2018

When your research requires consistent atmospheric control to maintain hypoxia, normoxia, or hyperoxia, you can count...

Coy anaerobic chambers #1 choice in microbiology

COY laboratory products

Oct 5, 2018

Used for over 40 years in anaerobic microbiology research, COY chambers are also critical to many other research areas...

ACEA Biosciences introduces an exploratory xCELLigence RTCA S16 instrument

Agilent technologies

Aug 30, 2018

The company has just introduced the xCELLigence RTCA S16, which is the ideal entry level model for exploratory studies...

X-RAD 160 qube

Precision X-Ray

Aug 23, 2018

The new X-RAD 160 qube is suitable for either mounting on the lab bench or mobile on an optional wheeled cart, allowing...

IncuCyte S3 Cell-by-Cell analysis software

Sartorius

Jul 27, 2018

The IncuCyte S3 Live-Cell Analysis System, "Cell-by-Cell Analysis" software module, and IncuCyte reagents provide a...

xCELLigence AccuWound 96 - Is your Wound Healing assay due for an upgrade?

Agilent technologies

Jul 26, 2018

Cell migration and invasion are critical to many biological fields. Scratch assay with AccuWound 96 brings...

Webinar Tuning Microenvironment to Control Cells for Improved Disease Modeling & Immunotherapy Work

Xcell Biosciences

Jul 16, 2018

The company producing Avatar, xCELL biosciences have organized a webinar about the tuning of microenvironment to...

Real-time verification of clonality using IncuCyte system

Sartorius

Jun 28, 2018

Use whole-well, label-free imaging to automatically scan for clones and verify monoclonality. Integrated image...

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Cancer Immunotherapy Applications of xCELLigence Real-Time Cell Analysis (RTCA) Systems

Mar 3, 2016

Many in vitro assays have been developed to screen and evaluate the efficacy of immune cell-mediated killing. The most common of these is the release assay where effector cell-mediated disruption of the target cell membrane results in leakage of its cytoplasmic contents into the culture medium. Endogenous biomolecules (such as lactate dehydrogenase) or previously added exogenous labels (such as the radioisotopes 51Cr or 111In) that leak into the media are then measured as an indirect readout of the damage caused by effector cells.

Alternative endpoint methods include flow cytometry, ELISA-based granzyme measurement, and morphometric analyses by microscopy. Although traditional end-point assays still serve as important tools for evaluating the efficacy of immune cell-mediated killing, there is a critical need for more efficient, homogeneous, automated assays (requiring less hands-on time) that aren’t hampered by the artifacts and hassle of using labels and that can provide quantitative results.

ACEA’s xCELLigence Real-Time Cell Analysis (RTCA) instruments utilize gold microelectrodes embedded in the bottom of microtiter wells to non-invasively monitor cell status including cell number, shape/size and attachment. The major distinguishing features of this technology include enhanced sensitivity, the preclusion of labels and, importantly, kinetic measurement of cell health/behavior.

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Agilent technologies

ACEA manufactures xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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The RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

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Instrument can be integrated with automated liquid handling platforms or a robotic plate handler from different vendors

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