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Cardiomyocyte isolation using Cellenion’s cellenONE instrument

Cellenion

Mar 24, 2023

The cellenONE platform is ideal for the isolation of fragile and heterogeneous cell size populations, such as...

Introducing Cytek Human Leukocyte Kit, the first ever 15-color lyse no-wash assay

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This kit has been designed to enumerate all major leukocyte subsets and it mirrors and expands on those identified in a...

Immunofluorescence Imaging for Rare Cell Detection with CyteFinder II

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CyteFinder II Instruments are high speed, whole slide imaging systems with options for liquid biopsy analysis and...

CellRad+ benchtop X-Ray irradiator

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New CellRad+ benchtop irradiator for biological samples is coming with higher voltage and continue exposure for 180...

Introducing Cytek Cloud

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Cytek Cloud is the new digital ecosystem that supports full spectrum flow cytometry research from panel design to data...

See More, Sort More with Cytek Full Spectrum Systems

Cytek Biosciences

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Still wondering whether to join the shift to full spectrum cytometry?  Easily transfer assays from your Cytek Northern...

Introducing MARS system : revolutionary solution for your workflow of cell separation and enrichment

Applied Cells

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The MARS family of instruments provides a breakthrough solution to complete the workflow of cell separation and...

Introducing RareCyte Orion, integrating Spatial Biology with Multiplex Imaging

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Orion, the highly multiplexed fluorescent imaging system, empower investigators to unlock spatial context for key...

xCELLigence eSight - Imaging & Impedance in real-time inside your incubator

Agilent technologies

Aug 22, 2022

The xCELLigence RTCA eSight enables comprehensive insight into cell health, cell behavior, cell function and cell...

Standardize tumor sample preparation and reduce background with Curiox laminar wash system

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Cancer Immunotherapy Applications of xCELLigence Real-Time Cell Analysis (RTCA) Systems

Mar 3, 2016

Many in vitro assays have been developed to screen and evaluate the efficacy of immune cell-mediated killing. The most common of these is the release assay where effector cell-mediated disruption of the target cell membrane results in leakage of its cytoplasmic contents into the culture medium. Endogenous biomolecules (such as lactate dehydrogenase) or previously added exogenous labels (such as the radioisotopes 51Cr or 111In) that leak into the media are then measured as an indirect readout of the damage caused by effector cells.

Alternative endpoint methods include flow cytometry, ELISA-based granzyme measurement, and morphometric analyses by microscopy. Although traditional end-point assays still serve as important tools for evaluating the efficacy of immune cell-mediated killing, there is a critical need for more efficient, homogeneous, automated assays (requiring less hands-on time) that aren’t hampered by the artifacts and hassle of using labels and that can provide quantitative results.

ACEA’s xCELLigence Real-Time Cell Analysis (RTCA) instruments utilize gold microelectrodes embedded in the bottom of microtiter wells to non-invasively monitor cell status including cell number, shape/size and attachment. The major distinguishing features of this technology include enhanced sensitivity, the preclusion of labels and, importantly, kinetic measurement of cell health/behavior.

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Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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The RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

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Instrument can be integrated with automated liquid handling platforms or a robotic plate handler from different vendors

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