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Enhance fluorescent signals of PE-conjugates using NovoCyte flow cytometer with 561nm laser

Aug 3, 2016

The advantages of using 561nm laser configured NovoCyte flow cytometers include: enhancing fluorescent signals of PE-conjugated antibodies with 561nm excitation and increasing sensitivity on detecting dim signals; eliminating or minimizing FITC to PE spillovers by independent excitation with 488nm and 561nm lasers; favoring the detection of multiple fluorescent proteins such as GFP, DsRed and mCherry; fulfilling multicolor analysis of variety of applications.

PE series of fluorochromes (PE, PE-Texas Red, PE-CF594, PE-Cy5, PE-Cy5.5 and PE-Cy7) have a primary absorption peak of 565nm with secondary absorption peaks at 496 and 545nm that can be excited by either the 488nm or 561nm lasers. However, higher excitation efficiency of 561nm laser results in brighter fluorescence signals and detection sensitivity, manifested by improved stain index and separation of positive from negative populations.

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Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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