Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
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Cytek full Spectrum flow Cytometry webinar: Part II – panel design and troubleshooting
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Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice
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Advances in Leukemia research using shear flow and Bioflux system
Dec 10, 2020
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Do you have our CellRad benchtop irradiator? It’s the only one on the market!
Dec 9, 2020
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Dosimetric characterization of an X-ray irradiator for use with cells
Dec 4, 2020
This study aims to characterize an X-ray irradiator for use with cells using its internal parallel-plate ionization...
Early immune response in mice immunized with inactivated vaccine against SARS-CoV-2
Nov 23, 2020
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Curiox - how to retain more cells for Single Cell Sequencing and proteogenomics ?
Nov 19, 2020
Do you think that centrifuging cells at 500 x g is not going to cause issues? Moreover, sample prep involving...
CS-FNX Hitachi Koki, discover the state-of-art ultracentrifuges series
Nov 19, 2020
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Enhance fluorescent signals of PE-conjugates using NovoCyte flow cytometer with 561nm laser
Aug 3, 2016
The advantages of using 561nm laser configured NovoCyte flow cytometers include: enhancing fluorescent signals of PE-conjugated antibodies with 561nm excitation and increasing sensitivity on detecting dim signals; eliminating or minimizing FITC to PE spillovers by independent excitation with 488nm and 561nm lasers; favoring the detection of multiple fluorescent proteins such as GFP, DsRed and mCherry; fulfilling multicolor analysis of variety of applications.
PE series of fluorochromes (PE, PE-Texas Red, PE-CF594, PE-Cy5, PE-Cy5.5 and PE-Cy7) have a primary absorption peak of 565nm with secondary absorption peaks at 496 and 545nm that can be excited by either the 488nm or 561nm lasers. However, higher excitation efficiency of 561nm laser results in brighter fluorescence signals and detection sensitivity, manifested by improved stain index and separation of positive from negative populations.
Related technologies: Conventional flow cytometry
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