Discover

Back to filter

Related topics

Pretargeted delivery of PI3K/mTOR small-molecule inhibitor–loaded nanoparticles

Spectral Instruments Imaging

Jun 23, 2021

Overactivation of the PI3K/mTOR signaling has been identified in non-Hodgkin’s lymphoma....

Top 10 Pharma companies have chosen CYTEK for their flow cytometry facility

Cytek Biosciences

Jun 21, 2021

6 of Top 10 Pharma Companies (Based on total group revenues) have chosen CYTEK - full...

Elevated NSD3 histone methylation activity drives squamous cell lung cancer

Spectral Instruments Imaging

Jun 8, 2021

This work identifies NSD3 as a principal 8p11-12 amplicon-associated oncogenic driver in...

A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic

Tonbo Biosciences

May 21, 2021

Tonbo Biosciences flow cytometry reagents & antibodies are manufactured with the highest...

IncuCyte webinar recording: How to measure Immune Cell Killing of Tumor Cells effectively

Sartorius

May 19, 2021

Immuno-oncology (IO) has transformed cancer treatment. The number of treatments in the IO...

White Paper: Immunophenotyping Rare Immune Cells with Laminar Wash AUTO System

Curiox

May 18, 2021

The Laminar Wash (LW) AUTO system consists of a wall-less plate and a laminar flow cell...

Show all topics (10)

Examining the kinetics of Neutrophil phagocytosis by flow cytometry

Nov 20, 2017

Two methods for measuring phagocytosis are routinely used, microscopy and flow cytometry. Both techniques measure the capability of phagocytic cell to ingest a fluorescent particle (labeled microorganisms or beads).  There are several advantages to using flow cytometry to measure phagocytosis. Microscopy requires time consuming manual counting of ingested particles, which limits the total number of cells analyzed. Conversely, flow cytometry can analyze thousands of cells per second facilitating statistical analysis while offering multi-parametric measurements for simultaneous evaluation of neutrophil features. Finally, neutrophils are easy to prime and activate by manipulation in the lab, which is a necessary process for microscopic analysis of phagocytosis. Flow cytometry allows the measurement of neutrophil phagocytosis in white blood cells without creating artifacts and therefore may be more representative of phagocytosis in vivo. In this application note, we measured neutrophil phagocytosis on the NovoCyte flow cytometer under various conditions and quantified the effect of a potent actin remodeling inhibitor, cytochalasin D.

Read more

Scientific paper
Application
Product news

Related technologies: Conventional flow cytometry

Brand profile

Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

The best in class personalized flow cytometer

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey