Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
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Cytek full Spectrum flow Cytometry webinar: Part II – panel design and troubleshooting
Jan 11, 2021
In this session, we will continue to look at full spectrum flow cytometry and look at considerations for fluorescence...
IncuCyte for in-vitro analysis of SARS-CoV-2 vaccine candidate
Jan 4, 2021
Watch this video to learn how researcher at Humane Genomics are utilizing the Incucyte platform for the in-vitro...
Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice
Dec 29, 2020
Radio-active sources have been used routinely for the preconditioning in humanized mouse models, but safety issues have...
Coming in spring 2021, meet the Cytek Aurora CS
Dec 18, 2020
Ever wanted to take your full spectrum panel from your Cytek Aurora or Northern Lights cytometer and sort? Soon, you...
Advances in Leukemia research using shear flow and Bioflux system
Dec 10, 2020
Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes create disruptions in the immune...
Do you have our CellRad benchtop irradiator? It’s the only one on the market!
Dec 9, 2020
The CellRad is a smaller, simpler, safer, and more cost effective alternative to radioisotope or high-powered X-ray...
Dosimetric characterization of an X-ray irradiator for use with cells
Dec 4, 2020
This study aims to characterize an X-ray irradiator for use with cells using its internal parallel-plate ionization...
Early immune response in mice immunized with inactivated vaccine against SARS-CoV-2
Nov 23, 2020
The development of a vaccine against COVID-19 is a hot topic for many research laboratories all over the world. Our aim...
Curiox - how to retain more cells for Single Cell Sequencing and proteogenomics ?
Nov 19, 2020
Do you think that centrifuging cells at 500 x g is not going to cause issues? Moreover, sample prep involving...
CS-FNX Hitachi Koki, discover the state-of-art ultracentrifuges series
Nov 19, 2020
Hitachi Koki, as a world leading producer of centrifuges offers general purpose floor standing and table top...
Measurement of cell proliferation using NovoCyte flow cytometer
Nov 14, 2016
There are two approaches to studying cell proliferation. One is to observe changes in the cell cycle; the other is to follow the number of cell divisions over a period of time. In the first method, at the most, three cell divisions can be followed. We can measure proliferation through absolute cell counts or with a dye, such as CFSE. When cells labeled with CFSE divide, the dye is partitioned equally between daughter cells and we can measure the loss of CFSE fluorescence over time as the dye is continuously diluted. The mean fluorescence intensity (MFI) of the dye was also plotted with cell concentration over time to show the inverse relationship between the two. This type of assay is often used to look at changes in T lymphocyte activation.
Related technologies: Conventional flow cytometry
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