Discover

Back to filter

Related topics

Exosome-Mediated Delivery of Small Molecules, RNA & DNA for Development of Novel Cancer Therapeutics

Spectral Instruments Imaging

Jun 3, 2024

Disha Moholkar of University of Louisville's Gupta Lab
Tuesday, June 11, 2024, 6:30 PM
...

Have you missed any recent Cytek webinars? Watch webinars and product tutorial videos at any time

Cytek Biosciences

Apr 23, 2024

Have you missed any recent Cytek webinars? Watch webinars and product tutorial videos at any time at Cytek website....

Multiplexed tissue imaging using the Orion platform to reveal the Spatial Biology of Cancer

RareCyte

Mar 27, 2024

In this webinar Prof. Sandro Santagata, will reveal how Orion high-plex imaging and the use of this data, is valuable...

Improve your data resolution with Cytek Autofluorescence Extraction tool!

Cytek Biosciences

Feb 5, 2024

Samples with high autofluorescence (AF) can be difficult to resolve with conventional flow cytometry, especially if...

Webinar: transforming Calcium Flux analysis with Cytek Full Spectrum Profiling Technology

Cytek Biosciences

Jan 4, 2024

The research described in this webinar harnesses the innovative capabilities of FSP technology to perform advanced...

Webinar recording: BioFlux, the gold standard for Platelet Biology and Vascular Pharmacology studies

Cell Microsystems

Jan 3, 2024

The BioFlux system give you the ability to introduce physiological shear flow to your research and drug discovery...

Integration of Full Spectrum Analysis and Cell Sorting technology to propel discovery

Cytek Biosciences

Dec 19, 2023

In this on-demand Cytek webinar, Dr. Maria Jaimes discusses the fundamentals of cell sorting, including cell recovery,...

Preclinical In Vivo Optical Imaging

Spectral Instruments Imaging

Dec 11, 2023

Recorded webinar

Vizgen webinar: spatial relationships in Developmental Biology

Vizgen

Nov 21, 2023

MERFISH integrates spatial transcriptomics technology with high resolution spatial imaging, In this webinar we will...

ACCELA educational webinar: Drive key insights and discoveries with new live cell analysis applications for eSight

Agilent technologies

Nov 14, 2023

The xCELLigence RTCA eSight enables simultaneous real time biosensor impedance-based and...

Show all topics (10)

Optimization of (CAR) T cell design guided by sensitive sssessment of B cell killing

Jul 10, 2017

Wednesday, July 12, 2017, 9 pm CEST.

Dan MacLeod Ph.D., Precision BioSciences, Inc.  

At Precision BioSciences, we have developed an allogeneic method using our proprietary ARCUS gene-editing technology to generate “universal” CAR T cells from healthy donors. These cells are engineered to eliminate expression of the endogenous T cell receptor making them safe for transfer to unrelated patients. By integrating the CAR transgene at a single genomic site, we can tightly control the level of CAR expression on the cell surface. We further optimize the cells by designing new CAR T constructs that include modifications to the extracellular binding domains as well as the intracellular signaling domains. Evaluating the impact of these new modifications requires sensitive methods to assay CAR T cell function. Traditional methods for evaluating cytotoxicity cannot effectively predict in vivo activity of a CAR T product; this is because they assess short-term killing potential rather than the ability to expand, persist, and exhibit serial killing. We have used the ACEA xCELLigence instrument for both B Cell Killing Assays and Leukemic Cell Killing Assays to test multiple CAR constructs with modified ectodomains, endodomains, and other modifications, and we have found this platform sensitive enough to detect subtle yet significant differences in performance not observed in traditional killing assays. Furthermore, the sensitivity of the instrument has enabled us to evaluate CAR constructs using cell numbers that are orders of magnitude lower than other assays, greatly enhancing our throughput and capabilities.

Register now

Webinar

Brand profile

Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

The RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

show detail

Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

show detail

Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

show detail

Instrument can be integrated with automated liquid handling platforms or a robotic plate handler from different vendors

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey