Discover

Back to filter

Related topics

Intoducing cellenONE: The revolutionary Single Cell isolator

Cellenion

May 25, 2022

A revolutionary platform based on sciDROP PICO precision dispensing technology and coupled with advanced image...

Advances in Leukemia research using shear flow and Bioflux system

Fluxion Biosciences

May 24, 2022

Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes create disruptions in the immune...

Why use Cytek Aurora?

Cytek Biosciences

May 4, 2022

A prodigy incorporating a unique combination of patent-pending innovative technologies that takes flow cytometry to the...

Introducing Celloger Mini Plus: Automated and compact Live cell imaging inside your incubator

Curiosis

Apr 11, 2022

We are happy to introduce new Celloger Mini Plus, automated live cell imaging system! Click the video below to find out...

CQ1 live-cell confocal system: revealing the pathophysiology of COVID-19 infection

Yokogawa

Mar 21, 2022

Yokogawa decided to support the world’s fight against COVID-19, scientifically cooperating with research facilities...

In-vivo webinar: a versatile use of SPECT/CT in the development of theranostic tracers

MOLECUBES

Mar 9, 2022

Combining (preclinical) imaging and targeted therapy, is the basis of theranostics. In this webinar, Dr. Florea will...

Introducing IsoPlexis Single-Cell Natural Killer Panel

IsoPlexis

Mar 4, 2022

Because of their ability to kill tumour cells, NK cells are an attractive target in cancer immunotherapy, therapeutic...

Enhanced small particle detection on CytekAurora and Northern Lights

Cytek Biosciences

Mar 2, 2022

Aurora system with ESP option, can  fully resolve particles around 70nm and from the 80nm PS bead. The new violet laser...

Quantitative 3D Optical Imaging for Lago

Spectral Instruments Imaging

Feb 21, 2022

Spectral Instrument Imaging just announced a new, strategic partnership with InVivo Analytics, which enables...

Molecular imaging study design basics

Spectral Instruments Imaging

Jan 31, 2022

By combining study design, study management, and image analytics in all major imaging modalities, BioLaurus has...

Show all topics (10)

Quantifying the impact of a CD19-BiTE combining flow cytometry & real-time cell analysis

Jun 9, 2017

The ability to quantitatively correlate T cell biomarker expression/secretion with  target cell killing is critical in both basic and applied studies of tumor immunology. Coupling flow cytometry with multiplex bead-based immunoassays enables quantitative measurement of multiple secreted targets in a single sample simultaneously. In this Application Note, we demonstrate a workflow involving the use of an xCELLigence Real-Time Cell Analysis (RTCA) instrument in conjunction with a NovoCyte flow cytometer to study both CTL-mediated destruction of tumor cells and the corresponding secretion of cytokines and cytolytic proteins by CTLs. Representing a promising new class of therapeutics, bispecific T-cell engagers (BiTEs) harness the power of the adaptive immune response by enhancing the ability of CTLs to specifically recognize and eliminate tumors. For example, CD19-BiTEs  are designed to simultaneously bind CD3 on CTLs and CD19 on cells of B cell lineage, thereby enhancing the efficiency of CTL effector functions against various B cell derived tumors. Herein, the enhancement effects of CD19-BiTEs have been evaluated using three distinct assays. While target cell death was monitored using an RTCA impedance assay and a flow cytometry-based cytolysis assay, secretion of cytokines and cytolytic proteins was quantified in a bead-based multiplex flow cytometry assay.

Read more

Scientific paper
Application
Product news

Related technologies: Conventional flow cytometry

Brand profile

Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

The best in class personalized flow cytometer

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey