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Automatic, Real Time Acquisition of Bioluminescent Kinetic Curves

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Jun 27, 2024

Watch this pre-recorded webinar with Dr. Andrew Van Praagh to learn how our new Aura software feature —Kinetics—...

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RareCyte Orion is a benchtop, high resolution, whole slide multimodal imaging instrument. A combination of quantitative...

New release now available: Cytek Amnis AI v3.0 Software

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Automated Purification of Viral DNA and RNA from Biological Samples usingZymo Research Quick-DNA/RNA

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The precision of temperature control, efficient heating and cooling rates, and excellent temperature homogeneity across...

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In this webinar, we will describe a new innovative approach we developed that resolves these limitations. The method...

X-RAD 320 for irradiation therapy during quantifying study for in vivo collagen reorganization

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Exosome-Mediated Delivery of Small Molecules, RNA & DNA for Development of Novel Cancer Therapeutics

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Disha Moholkar of University of Louisville's Gupta Lab
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Emulate in vivo conditions – introduce shear flow to your experiments with BioFlux system

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Quantifying the impact of a CD19-BiTE combining flow cytometry & real-time cell analysis

Jun 9, 2017

The ability to quantitatively correlate T cell biomarker expression/secretion with  target cell killing is critical in both basic and applied studies of tumor immunology. Coupling flow cytometry with multiplex bead-based immunoassays enables quantitative measurement of multiple secreted targets in a single sample simultaneously. In this Application Note, we demonstrate a workflow involving the use of an xCELLigence Real-Time Cell Analysis (RTCA) instrument in conjunction with a NovoCyte flow cytometer to study both CTL-mediated destruction of tumor cells and the corresponding secretion of cytokines and cytolytic proteins by CTLs. Representing a promising new class of therapeutics, bispecific T-cell engagers (BiTEs) harness the power of the adaptive immune response by enhancing the ability of CTLs to specifically recognize and eliminate tumors. For example, CD19-BiTEs  are designed to simultaneously bind CD3 on CTLs and CD19 on cells of B cell lineage, thereby enhancing the efficiency of CTL effector functions against various B cell derived tumors. Herein, the enhancement effects of CD19-BiTEs have been evaluated using three distinct assays. While target cell death was monitored using an RTCA impedance assay and a flow cytometry-based cytolysis assay, secretion of cytokines and cytolytic proteins was quantified in a bead-based multiplex flow cytometry assay.

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Related technologies: Conventional flow cytometry

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Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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