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Cytek full Spectrum flow Cytometry webinar: Part II – panel design and troubleshooting

Cytek Biosciences

Jan 11, 2021

In this session, we will continue to look at full spectrum flow cytometry and look at considerations for fluorescence...

IncuCyte for in-vitro analysis of SARS-CoV-2 vaccine candidate

Sartorius

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Watch this video to learn how researcher at Humane Genomics are utilizing the Incucyte platform for the in-vitro...

Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice

Precision X-Ray

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Radio-active sources have been used routinely for the preconditioning in humanized mouse models, but safety issues have...

Advances in Leukemia research using shear flow and Bioflux system

Fluxion Biosciences

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Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes create disruptions in the immune...

Dosimetric characterization of an X-ray irradiator for use with cells

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This study aims to characterize an X-ray irradiator for use with cells using its internal parallel-plate ionization...

Curiox - how to retain more cells for Single Cell Sequencing and proteogenomics ?

Curiox

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Do you think that centrifuging cells at 500 x g is not going to cause issues? Moreover, sample prep involving...

CS-FNX Hitachi Koki, discover the state-of-art ultracentrifuges series

Hitachi Koki

Nov 19, 2020

Hitachi Koki, as a world leading producer of centrifuges offers general purpose floor standing and table top...

Discover the new NovoCyte Penteon - when exceptional performance meets simplicity

Agilent technologies

Nov 18, 2020

The NovoCyte Penteon flow cytometer builds on its successful predecessor, the NovoCyte. You now have the flexibility to...

Cellular stress induces erythrocyte assembly and promotes microangiopathy

Fluxion Biosciences

Dec 19, 2018

Microangiopathy with subsequent organ damage represents a major complication in several diseases. The mechanisms...

The need for ultra high field magnetic resonance imaging

Bruker Biospin

Dec 17, 2018

MRI  is a noninvasive imaging technology that is known for its superior soft tissue contrast and ability to provide...

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Quantitative measurements of apoptosis using the ACEA NovoCyte Flow Cytometer

Feb 23, 2016

In order to demonstrate the flexibility and verstality of the NovoCyte system for assessment of apoptotic events, we show three different assays that can measure apoptosis in response to staurosporine, a broad kinase inhibitor known to induce apoptosis in many cell types.

The first assay uses Annexin V to bind exposed PS on cell membranes for labeling early apoptotic cells and the DNA binding dye, 7-AAD, for labeling late apoptotic cells. 

The second assay makes use of the fluorescent dye JC-1, to measure the loss of mitochondrial membrane potential in early apoptotic cells. JC-1 is cell permeable and aggregates inside mitochondria when membrane potential is maintained, emitting fluorescence in the PE channel.

The third assay quantifies caspase activity. Probe molecules containing a cleavage site specic for caspase 3 & 7 are added to cells. When cleaved by these activated caspases in early apoptotic cells, the probe molecules enter the nucleus, bind to DNA, and fluoresce. This enables the direct readout of activated caspases in cells.

Read the application note.

Application

Related technologies: Conventional flow cytometry

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Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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