Discover

Back to filter

Related topics

Recent publication of in-vivo two-photon intravital imaging study targeting mouse kidney

IVIM Technology

May 22, 2023

In vivo longitudinal 920 nm two-photon intravital kidney imaging of a dynamic 2,8-DHA crystal
formation and
...

Webinar: Multimodal tissue imaging and machine learning to advance precision medicine

RareCyte

May 17, 2023

Join us for this webinar to learn how the Orion spatial biology imaging platform was used to identify prognostic...

Mantis ROI calculator

Formulatrix

May 5, 2023

Decision to improve the output in a lab is always taken based on knowledge and workflow needs. But final decision to...

Real-Time and Quantitative Analysis of Macrophage Phagocytosis with RTCA eSight

Agilent technologies

May 4, 2023

The eSight is currently the only instrument that interrogates cell health and behavior using cellular
impedance
...

Thermal Shift Assay using SYPRO Orange to Detect Protein Melting Temperatures

Analytik Jena

May 2, 2023

The thermal shift assay is based on temperature-induced denaturation and can be monitored using SYPRO Orange. This...

A deep learning and Monte Carlo based framework for bioluminescence imaging center Maastro

Precision X-Ray

Apr 20, 2023

"In this paper,we developed a framework using deep learning for bioluminescence-based targeting for GBM animal...

Gentle sorting of microbial cells and sub-micron particles using WOLF sorter

NanoCellect

Apr 18, 2023

While most modern applications of flow cytometry may focus on cells of eukaryotic origin, the first flow analyzers were...

InAlyzer to evaluate G6PD activity in relation with frailty

MEDIKORS

Mar 28, 2023

InAlyzer is body densitometry instrument for lab animals, equipped with 2 X-Ray sources and able to provide valuable...

Cardiomyocyte isolation using Cellenion’s cellenONE instrument

Cellenion

Mar 24, 2023

The cellenONE platform is ideal for the isolation of fragile and heterogeneous cell size populations, such as...

Recorded webinar: Anesthesia Considerations in Small Animal Imaging

Spectral Instruments Imaging

Mar 13, 2023

Anesthesia settings and operation of Optical Imaging Systems: methods, animal handling, safety and regulatory...

Show all topics (10)

Quantitative measurements of apoptosis using the ACEA NovoCyte Flow Cytometer

Feb 23, 2016

In order to demonstrate the flexibility and verstality of the NovoCyte system for assessment of apoptotic events, we show three different assays that can measure apoptosis in response to staurosporine, a broad kinase inhibitor known to induce apoptosis in many cell types.

The first assay uses Annexin V to bind exposed PS on cell membranes for labeling early apoptotic cells and the DNA binding dye, 7-AAD, for labeling late apoptotic cells. 

The second assay makes use of the fluorescent dye JC-1, to measure the loss of mitochondrial membrane potential in early apoptotic cells. JC-1 is cell permeable and aggregates inside mitochondria when membrane potential is maintained, emitting fluorescence in the PE channel.

The third assay quantifies caspase activity. Probe molecules containing a cleavage site specic for caspase 3 & 7 are added to cells. When cleaved by these activated caspases in early apoptotic cells, the probe molecules enter the nucleus, bind to DNA, and fluoresce. This enables the direct readout of activated caspases in cells.

Read the application note.

Application

Related technologies: Conventional flow cytometry

Brand profile

Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

The best in class personalized flow cytometer

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey