Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
Discover
Related topics
SARS-CoV-2 N Protein Antigen Test as new Quanterix tool in ultrasensitive biomarkers detection
Apr 12, 2021
Digital health solution, Simoa, is changing the way in which the biology of health and disease is studied by giving...
Single-cell DNA Analysis of a Novel Acute Erythroid Leukemia CRISPR Induced Mouse Model
Apr 12, 2021
Single-cell DNA Analysis of a Novel Acute Erythroid Leukemia (AEL) CRISPR Induced Mouse Model.
Assessment of...
Developing tools for multiplex to single EV analysis pipelines
Apr 12, 2021
Extracellular vesicles (EVs) are lipid spheres released from cells. EVs express proteins...
SARS-CoV-2 detection workflow in wastewater- Analytik Jena and project in Japan
Apr 12, 2021
In 2020 Analytik Jenas real-time PCR thermal cyclers of the qTOWER³ series even supported one of the first SARS-CoV-2...
Mar 31, 2021
Cancer cells exhibit metabolic...
Cytek webinar : Improved flow cytometry assay resolution with autofluorescence extraction
Mar 30, 2021
The power of autofluorescence extraction in the detection of dim fluorescence on highly...
Innovation never takes a break with IncuCyte
Mar 11, 2021
Analyze your cells for days, weeks or even months as they sit stationary in the stable...
Curiox webinar - how to retain more cells for Single Cell Sequencing and proteogenomics ?
Feb 15, 2021
Curiox Laminar Wash technology has been added as part of the official BioLegend TotalSeq...
Cytek Aurora in Action : how to easily resolve highly overlapping dyes ?
Feb 2, 2021
Learn from Jeff Nelson, a manager of a cytometry shared resource laboratory, about the...
Webinar: A simple Migration/Invasion workflow using IncuCyte Live-cell analysis system
Jan 27, 2021
Cell migration and invasion, or cell motility, play a role in many normal and...
Feb 23, 2016
In order to demonstrate the flexibility and verstality of the NovoCyte system for assessment of apoptotic events, we show three different assays that can measure apoptosis in response to staurosporine, a broad kinase inhibitor known to induce apoptosis in many cell types.
The first assay uses Annexin V to bind exposed PS on cell membranes for labeling early apoptotic cells and the DNA binding dye, 7-AAD, for labeling late apoptotic cells.
The second assay makes use of the fluorescent dye JC-1, to measure the loss of mitochondrial membrane potential in early apoptotic cells. JC-1 is cell permeable and aggregates inside mitochondria when membrane potential is maintained, emitting fluorescence in the PE channel.
The third assay quantifies caspase activity. Probe molecules containing a cleavage site specic for caspase 3 & 7 are added to cells. When cleaved by these activated caspases in early apoptotic cells, the probe molecules enter the nucleus, bind to DNA, and fluoresce. This enables the direct readout of activated caspases in cells.
Related technologies: Conventional flow cytometry
Brand profile
Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
More info at:
www.aceabio.com