Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
Discover
Back to filter
Show all topics (10)
Related topics
Intoducing cellenONE: The revolutionary Single Cell isolator
May 25, 2022
A revolutionary platform based on sciDROP PICO precision dispensing technology and coupled with advanced image...
Advances in Leukemia research using shear flow and Bioflux system
May 24, 2022
Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes create disruptions in the immune...
May 4, 2022
A prodigy incorporating a unique combination of patent-pending innovative technologies that takes flow cytometry to the...
In-vivo webinar: a versatile use of SPECT/CT in the development of theranostic tracers
Mar 9, 2022
Combining (preclinical) imaging and targeted therapy, is the basis of theranostics. In this webinar, Dr. Florea will...
Introducing IsoPlexis Single-Cell Natural Killer Panel
Mar 4, 2022
Because of their ability to kill tumour cells, NK cells are an attractive target in cancer immunotherapy, therapeutic...
Enhanced small particle detection on CytekAurora and Northern Lights
Mar 2, 2022
Aurora system with ESP option, can fully resolve particles around 70nm and from the 80nm PS bead. The new violet laser...
Quantitative 3D Optical Imaging for Lago
Feb 21, 2022
Spectral Instrument Imaging just announced a new, strategic partnership with InVivo Analytics, which enables...
Molecular imaging study design basics
Jan 31, 2022
By combining study design, study management, and image analytics in all major imaging modalities, BioLaurus has...
Extensive assessment of Cytokine production on the NovoCyte Penteon flow cytometer
Nov 29, 2021
Cytokines are small molecules essential for immune cell response to activation by pathogens, autoimmunity, or...
Accelerating AML Research Through Single-cell Multi-omics- integrate seamlessly into existing NGS
Nov 25, 2021
Dr. Linde Miles Memorial Sloan Kettering Cancer Center about her research, use of the Tapestri as a single-cell...
Quantitative measurements of apoptosis using the ACEA NovoCyte Flow Cytometer
Feb 23, 2016
In order to demonstrate the flexibility and verstality of the NovoCyte system for assessment of apoptotic events, we show three di fferent assays that can measure apoptosis in response to staurosporine, a broad kinase inhibitor known to induce apoptosis in many cell types.
The fi rst assay uses Annexin V to bind exposed PS on cell membranes for labeling early apoptotic cells and the DNA binding dye, 7-AAD, for labeling late apoptotic cells.
The second assay makes use of the fl uorescent dye JC-1, to measure the loss of mitochondrial membrane potential in early apoptotic cells. JC-1 is cell permeable and aggregates inside mitochondria when membrane potential is maintained, emitting fluorescence in the PE channel.
The third assay quantifi es caspase activity. Probe molecules containing a cleavage site speci c for caspase 3 & 7 are added to cells. When cleaved by these activated caspases in early apoptotic cells, the probe molecules enter the nucleus, bind to DNA, and fluoresce. This enables the direct readout of activated caspases in cells.
Read the application note.
Related technologies: Conventional flow cytometry
Brand profile
