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Introducing the xCELLigence Immunotherapy kit – B Cell killing assay

Jul 28, 2016

ACEA’s xCELLigence Real-Time Cell Analysis (RTCA) instruments utilize gold microelectrodes embedded in the bottom of microtiter wells to non-invasively monitor cell number, cell size/shape, and cell-substrate attachment strength. By pre-coating these wells with an antibody that is specific for B cells, B lymphoblast cancers are immobilized and proliferate to the point of confluence. Upon exposure to diverse effectors (NK cell, T cells, CART, oncolytic virus, checkpoint inhibitors, etc.) a continuous kinetic trace of B cell destruction is automatically recorded by the xCELLigence instrument.

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Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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The RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

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