Discover

Back to filter

Related topics

FLO i8 - new liquid handler with 8 independently spanning channels

Formulatrix

Jun 1, 2023

The FLO i8 Liquid Handler is ideal for optimizing your protocols and bench work, allowing you to focus on the research,...

Precision Launches CellRadHD – The Next Generation High-Dose Benchtop Irradiator

Precision X-Ray

May 31, 2023

New CellRadHD offers the highest dose available in a benchtop unit. Up to 160kV, this irradiator is maximizing...

Novocyte 3005 installation in CEMEX Iasi, Romania

Agilent technologies

May 29, 2023

Presentation movie

Recent publication of in-vivo two-photon intravital imaging study targeting mouse kidney

IVIM Technology

May 22, 2023

In vivo longitudinal 920 nm two-photon intravital kidney imaging of a dynamic 2,8-DHA crystal
formation and
...

Webinar: Multimodal tissue imaging and machine learning to advance precision medicine

RareCyte

May 17, 2023

Join us for this webinar to learn how the Orion spatial biology imaging platform was used to identify prognostic...

Mantis ROI calculator

Formulatrix

May 5, 2023

Decision to improve the output in a lab is always taken based on knowledge and workflow needs. But final decision to...

Real-Time and Quantitative Analysis of Macrophage Phagocytosis with RTCA eSight

Agilent technologies

May 4, 2023

The eSight is currently the only instrument that interrogates cell health and behavior using cellular
impedance
...

Revolutionize your Flow Cytometry and Sorting workflow with Cytek Biosciences

Cytek Biosciences

May 3, 2023

Using full spectrum flow cytometry, Cytek systems ( RUO and CE-IVD) detect the entire fluorochrome emission, allowing...

Thermal Shift Assay using SYPRO Orange to Detect Protein Melting Temperatures

Analytik Jena

May 2, 2023

The thermal shift assay is based on temperature-induced denaturation and can be monitored using SYPRO Orange. This...

A deep learning and Monte Carlo based framework for bioluminescence imaging center Maastro

Precision X-Ray

Apr 20, 2023

"In this paper,we developed a framework using deep learning for bioluminescence-based targeting for GBM animal...

Show all topics (10)

Monitoring in real time the formation and removal of biofilms from clinical related pathogens

Nov 25, 2016

The search for novel anti-biofilm bio-active molecules is a very active research area for which simple, reliable, and fast screening methods are demanded. In this work we have successfully validated an impedance-based method, initially developed for the study of adherent eukaryotic cells, to monitor the formation of single-species biofilms of three model bacteria in real time. The xCelligence real time cell analyzer (RTCA) equipment uses specific microtiter E-plates coated with gold-microelectrodes that detect the attachment of adherent cells, thus modifying the impedance signal. In the current study, this technology allowed the distinction between biofilm-producers and non-producers of Staphylococcus aureus and Staphylococcus epidermidis, as well as the formation of Streptococcus mutans biofilms only when sucrose was present in the culture medium. Besides, different impedance values permitted discrimination among the biofilm-producing strains tested regardless of the nature of the polymeric biofilm matrix. Finally, we have continuously monitored the inhibition of staphylococcal biofilm formation by the bacteriophage phi-IPLA7 and the bacteriophage-encoded endolysin LysH5, as well as the removal of a preformed biofilm by this last antimicrobial treatment. Results observed with the impedance-based method showed high correlation with those obtained with standard approaches, such as crystal violet staining and bacteria enumeration, as well as with those obtained upon other abiotic surface (polystyrene and stainless steel). Therefore, this RTCA technology opens new opportunities in the biofilm research arena and its application could be further explored for other bacterial genera as well as for different bio-active molecules.

Read more

Scientific paper
Application

Brand profile

Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

The RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

show detail

Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

show detail

Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

show detail

Instrument can be integrated with automated liquid handling platforms or a robotic plate handler from different vendors

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey