ACEA manufactures xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
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Real-time chemotaxis of macrophages
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Iqbal and colleagues (PLoS ONE. 2013 Mar; 8(3): e58744) used the xCELLigence RTCA DP system and CIM-Plate to quantify the migration of murine macrophages in response to CC chemokines and other chemoattractant signaling molecules.They stated that “the xCELLigence real-time migration assay offers a rapid and reproducible system to monitor macrophage chemotaxis,” and “quantification is also far more rapid and accurate compared to the modified Boyden chamber where multiple images are required followed by staining and counting which can also introduce operator bias”.
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