Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
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Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice
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Radio-active sources have been used routinely for the preconditioning in humanized mouse...
Advances in Leukemia research using shear flow and Bioflux system
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Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes...
Do you have our CellRad benchtop irradiator? It’s the only one on the market!
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Early immune response in mice immunized with inactivated vaccine against SARS-CoV-2
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Cellular stress induces erythrocyte assembly and promotes microangiopathy
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Microangiopathy with subsequent organ damage represents a major complication in several diseases. The mechanisms...
The need for ultra high field magnetic resonance imaging
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The most advanced software for preclinical imaging research
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Modern imaging laboratories hosting PET/MR instruments profit from the fully integrated common imaging platform...
Red blood cell-hitchhiking boosts delivery of nanocarriers to chosen organs
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Drug delivery by nanocarriers (NCs) has long been stymied by dominant liver uptake and limited target organ deposition,...
Adenovirus type B detection using the xxpress qPCR thermal cycler
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The ultrafast xxpress qPCR thermal cycler through performing standard dilution series and multiplex qPCR experiments is...
Real-time determination of virus titers using cell electric impedance technology
Apr 12, 2017
Vero cells in suspension were incubated with serial dilutions of a known concentration of West Nile virus (WNV) for 30 minutes at 37°C, followed by immediate addition of the cell/virus suspension to wells of ACEA’s electronic microtiter plates (E-Plates). In contrast to uninfected control cells which grew to confluency and maintained a plateaued Cell Index (CI), virus-infected cells displayed a time-dependent decrease of CI down to zero, indicating complete cell lysis. Importantly, the time at which this cytopathic effect occurred correlated extremely well with the known titer of the virus. This is highlighted by plotting the CIT50 (time required for the CI to decrease by 50%) as a function of virus titer. Using this type of standard curve, it is possible to determine the viral titer in samples of unknown concentration.
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