Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
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Outstanding post-sort sample recovery on the WOLF Cell Sorter
Jun 14, 2022
The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting...
Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters
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Favipiravir at high doses has potent antiviral activity in SARS-CoV-2−infected hamsters, whereas hydroxychloroquine...
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Feb 21, 2022
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Extensive assessment of Cytokine production on the NovoCyte Penteon flow cytometer
Nov 29, 2021
Cytokines are small molecules essential for immune cell response to activation by pathogens, autoimmunity, or...
How to Improve Your Viral Nucleic Acid Extraction Processes
Sep 6, 2021
High-Throughput Extraction with CyBio FeliX
Comparison of gamma and x-ray irradiation results
Aug 12, 2021
Biological effects of Cs-137 and X-ray irradiators are comparable with regards to...
Hypoxia further exacerbates myopathy in broilers via alteration of satellite cell fate
Aug 2, 2021
Woody breast (WB) condition has created a variety of challenges for the global poultry...
High quality Bioluminescence and X-Ray analysis in lung squamous cell carcinoma
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Pretargeted delivery of PI3K/mTOR small-molecule inhibitor–loaded nanoparticles
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Overactivation of the PI3K/mTOR signaling has been identified in non-Hodgkin’s lymphoma....
Quantitative two-photon microscopy imaging analysis of human skin
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Real-time determination of virus titers using cell electric impedance technology
Apr 12, 2017
Vero cells in suspension were incubated with serial dilutions of a known concentration of West Nile virus (WNV) for 30 minutes at 37°C, followed by immediate addition of the cell/virus suspension to wells of ACEA’s electronic microtiter plates (E-Plates). In contrast to uninfected control cells which grew to confluency and maintained a plateaued Cell Index (CI), virus-infected cells displayed a time-dependent decrease of CI down to zero, indicating complete cell lysis. Importantly, the time at which this cytopathic effect occurred correlated extremely well with the known titer of the virus. This is highlighted by plotting the CIT50 (time required for the CI to decrease by 50%) as a function of virus titer. Using this type of standard curve, it is possible to determine the viral titer in samples of unknown concentration.
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