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Real-time monitoring of Human Skin-Derived Precursor (SKPs) cell differentiation

Sep 2, 2016

There are two major classes of stem cells: pluripotent that can become any cell in the adult body, and multipotent that are restricted to becoming a more limited population of cells. Cell sources, characteristics, differentiation and therapeutic applications are discussed. Stem cells have great potential in tissue regeneration and repair but much still needs to be learned about their biology, manipulation and safety before their full therapeutic potential can be achieved.

In this assay, differentiation of human skin-derived precursors (SKPs) into smooth muscle cells (SMCSs) and undifferentiated SKPs with or without the application of transforming growth factor (TGF)-β1 or TGF-β3, and subsequently fixed and stained to display a-smooth actin, calponin, and SM22a. Real-time analysis using the xCELLigence RTCA system, correlates the increase in Cell Index with that of morphological differences between the differentiated and undifferentiated SKPs.

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Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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The RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

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