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Introducing the GenapSys Sequencer

GenapSys

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GenapSys has developed a novel electrical-based platform capable of accurately detecting single base incorporations....

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Microangiopathy with subsequent organ damage represents a major complication in several diseases. The mechanisms...

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MRI  is a noninvasive imaging technology that is known for its superior soft tissue contrast and ability to provide...

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Agilent technologies

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Cell proliferation is one of the most fundamental biological processes in biology. Many of the traditional assays to...

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Do you already heard the hypothesis that metabolic changes in the heart may precede and even trigger structural and...

IncuCyte neuronal activity assay

Sartorius

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The generation and maturation of neuronal networks are necessary characteristics of neurogenesis, providing...

The most advanced software for preclinical imaging research

Bruker Biospin

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Modern imaging laboratories hosting PET/MR instruments profit from the fully integrated common imaging platform...

COY - personalized hypoxic chambers

COY laboratory products

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When your research requires consistent atmospheric control to maintain hypoxia, normoxia, or hyperoxia, you can count...

Red blood cell-hitchhiking boosts delivery of nanocarriers to chosen organs

Fluxion Biosciences

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Drug delivery by nanocarriers (NCs) has long been stymied by dominant liver uptake and limited target organ deposition,...

Coy anaerobic chambers #1 choice in microbiology

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Used for over 40 years in anaerobic microbiology research, COY chambers are also critical to many other research areas...

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Real-time monitoring of Human Skin-Derived Precursor (SKPs) cell differentiation

Sep 2, 2016

There are two major classes of stem cells: pluripotent that can become any cell in the adult body, and multipotent that are restricted to becoming a more limited population of cells. Cell sources, characteristics, differentiation and therapeutic applications are discussed. Stem cells have great potential in tissue regeneration and repair but much still needs to be learned about their biology, manipulation and safety before their full therapeutic potential can be achieved.

In this assay, differentiation of human skin-derived precursors (SKPs) into smooth muscle cells (SMCSs) and undifferentiated SKPs with or without the application of transforming growth factor (TGF)-β1 or TGF-β3, and subsequently fixed and stained to display a-smooth actin, calponin, and SM22a. Real-time analysis using the xCELLigence RTCA system, correlates the increase in Cell Index with that of morphological differences between the differentiated and undifferentiated SKPs.

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Agilent technologies

ACEA manufactures xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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The RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

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