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Understand the Cancer Ecosystem based on single cells analysis

Mission Bio

Sep 22, 2022

Mission Bio is a life sciences company that accelerates discoveries and cures for a wide range of diseases by equipping...

Automating DNA Extraction for High-Throughput Bacterial Plasmid Purification with CyBi®-SELMA

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The Need to Increase Plasmid Isolation Throughput, Challenges with Scaling up Plasmid DNA Extraction, Automating the...

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Cytek Aurora at CIVIS Summer School

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xCELLigence eSight - Imaging & Impedance in real-time inside your incubator

Agilent technologies

Aug 22, 2022

The xCELLigence RTCA eSight enables comprehensive insight into cell health, cell behavior, cell function and cell...

Standardize tumor sample preparation and reduce background with Curiox laminar wash system

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Laminar Wash technology offers a gentle alternative that can improve yield and data quality, particularly of rare cell...

Developing a 43 color panel on Cytek Aurora using Laminar Wash for sample prep

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Successfully executing a 43 color panel is a technically demanding task. One that is made more difficult when there are...

Transcriptome-Informed Approach to Drug Discovery

HTG Molecular Diagnostics

Jul 12, 2022

The HTG EdgeSeq system is a powerful molecular profiling tool for biopharmaceutical companies, academic research...

Semi-automated genomic DNA extractionfrom Plant with CyBi®-SELMA

Analytik Jena

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Plant genomic DNA extraction with CyBio SELMA, Semi-Automated Pipette with minimal benchtop footprint

New Center of Excellence, located at the Josep Carreras Leukaemia Research Institute

Mission Bio

Jul 12, 2022

Mission Bio is a life sciences company that accelerates discoveries and cures for a wide range of diseases by equipping...

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Real-time monitoring of Human Skin-Derived Precursor (SKPs) cell differentiation

Sep 2, 2016

There are two major classes of stem cells: pluripotent that can become any cell in the adult body, and multipotent that are restricted to becoming a more limited population of cells. Cell sources, characteristics, differentiation and therapeutic applications are discussed. Stem cells have great potential in tissue regeneration and repair but much still needs to be learned about their biology, manipulation and safety before their full therapeutic potential can be achieved.

In this assay, differentiation of human skin-derived precursors (SKPs) into smooth muscle cells (SMCSs) and undifferentiated SKPs with or without the application of transforming growth factor (TGF)-β1 or TGF-β3, and subsequently fixed and stained to display a-smooth actin, calponin, and SM22a. Real-time analysis using the xCELLigence RTCA system, correlates the increase in Cell Index with that of morphological differences between the differentiated and undifferentiated SKPs.

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Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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The RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

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Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

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