Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
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xCELLigence cancer immunotherapy: NK cell-mediated cytolysis
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ACEA Biosciences developed a label-free assay format that allows dynamic monitoring of NK cell-mediated cytolysis. The assay uses impedancebased technology (RT-CES)4,5. This system is the predecessor of the new xCELLigence System. The attachment and interaction of adherent cells with wells on a 96X E-Plate leads to impedance changes, which correlate with cell number, size and shape. In contrast, addition of suspended cells to the wells results in undetectable changes due to non-existent or weak interaction with the electrodes. Therefore, NK cell-mediated cytolytic effects on the cancer cell monolayer can be quantitatively and dynamically monitored on the sensor plate without labeling the target cells.
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